led光源在园艺与药用植物和人工虫草室内培养中的应用

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1、LED光源在园艺与药用植物和人工虫草室内培养中的应用华南师范大学生命科学学学院李海航,博士、教授、博导2014年6月21日，广州广东省植物生长调节剂协会与广州市果树科学研究所2014年植物生长调节剂与现代农业研讨会植物的种子萌发、幼苗生长、形态建成、开花结果等过程都需要光不同的生物及不同生长发育期需要不同的光质与光强降温光在植物与食用菌等的室内培养中广泛应用光照研究内容非洲菊组织培养不定芽诱导、芽增殖和生根铁皮石斛和金钗石斛组培繁殖原球茎增殖、生长、分化虫草子实体形成,生长,功能成分子实体的诱导形成生长：高、鲜重、干重生物活性物质:虫草素,多糖,类胡萝卜素Explant:Dedif

2、ferentiationcallusRedifferentiationSingleshootClustershootsRootingPlantletsMultiplicationDivisionAdventitiousbudsLEDtreatmentShootmultiplicationprocedureComparedtoWFL,nosignificantdifferenceswereobservedinthetreatmentsofV1,V2,V5andV6.V3andV4decreasedtheFWoftheshoots.V6increaseddryweightsignifi

3、cantly.FreshweightanddryweightHeightandnumberofshootsAllLEDscouldincreasetheheightofshootsexceptV5.ButNo.ofshoots/explanthadnosignificantdifferent.ChlorophyllcontentAllLEDsinducedchlorophyllbiosynthesissignificantly,comparedwithWFLWV1V2V3V4V5V6WFL35daysculturedinvitrounderLEDV1V4V3V6V2V5WFL35d

4、aysculturedinvitrounderLEDExplant:DedifferentiationcallusRedifferentiationRootingPlantletsResults—RootinductionReceptacleMultiplicationDivisionAdventitiousbudsClustershootsRootinductionunderLEDSingleshootV3treatmentdecreasedFWandDW.V2treatmentincreasedFWandDWsignificantly.FreshweightandDryweig

5、htHeightsofshootsunderV1,V2,V3andV4weresignificantlyhigherthancontrol.V2hadthemostnumberofrootscomparedwithothers.ShootheightandrootnumberV3,V4andV5promotedtherootactivitysignificantly.V2increasedthemaximumrootlength.RootactivityandmaximumrootlengthV1V2V3V4V5V6WFLSeedlings35dafterrootinginduct

6、ionSurvivalrateHeight(cm)No.ofleavesV1V2V3V4V5V6WFL100%100%100%100%100%100%100%9.46±0.22a9.77±0.22a8.19±0.34b9.54±0.30a8.02±0.27b8.25±0.48b7.09±0.23c5.19±0.23b4.94±0.11b6.06±0.19a6.00±0.35a5.31±0.24b6.19±0.20a4.60±0.27bResults—Transplantation15daysaftertransplantation,nodifferenceonseedlingssu

7、rvivalrates.AllLEDtreatedseedlingsweresignificanthigherandnumberofleavesincreased.V4V1V5V2V6V3WFLTransplantedseedlingsgrownfor15days•Among6LEDs,V1,V2andV6weresuitableforshootmultiplicationandV2wasgoodforrootinduction.•Survivalratesofsee