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1、MacromolecularCrystallographyThispageintentionallyleftblankMacromolecularCrystallographyconventionalandhigh-throughputmethodsEDITEDBYMarkSandersonandJaneSkelly13GreatClarendonStreet,OxfordOX26DPOxfordUniversityPressisadepartmentoftheUniversityofOxford.ItfurtherstheUniversity’sobjectiveofexc
2、ellenceinresearch,scholarship,andeducationbypublishingworldwideinOxfordNewYorkAucklandCapeTownDaresSalaamHongKongKarachiKualaLumpurMadridMelbourneMexicoCityNairobiNewDelhiShanghaiTaipeiTorontoWithofficesinArgentinaAustriaBrazilChileCzechRepublicFranceGreeceGuatemalaHungaryItalyJapanPolandPor
3、tugalSingaporeSouthKoreaSwitzerlandThailandTurkeyUkraineVietnamOxfordisaregisteredtrademarkofOxfordUniversityPressintheUKandincertainothercountriesPublishedintheUnitedStatesbyOxfordUniversityPressInc.,NewYork©OxfordUniversityPress,2007ThemoralrightsoftheauthorshavebeenassertedDatabaserightO
4、xfordUniversityPress(maker)Firstpublished2007Allrightsreserved.Nopartofthispublicationmaybereproduced,storedinaretrievalsystem,ortransmitted,inanyformorbyanymeans,withoutthepriorpermissioninwritingofOxfordUniversityPress,orasexpresslypermittedbylaw,orundertermsagreedwiththeappropriatereprog
5、raphicsrightsorganization.EnquiriesconcerningreproductionoutsidethescopeoftheaboveshouldbesenttotheRightsDepartment,OxfordUniversityPress,attheaddressaboveYoumustnotcirculatethisbookinanyotherbindingorcoverandyoumustimposethesameconditiononanyacquirerBritishLibraryCataloguinginPublicationDa
6、taDataavailableLibraryofCongressCataloginginPublicationDataDataavailableTypesetbyNewgenImagingSystems(P)Ltd.,Chennai,IndiaPrintedinGreatBritainonacid-freepaperbyAntonyRowe,Chippenham,WiltshireISBN978–0–19–852097–910987654321PrefaceThenatureofmacromolecularcrystallographyhas(Chapters14and15)
7、,viruses,andmembranepro-changedgreatlyoverthepast10years.Increas-teins(Chapter16).Ourabilitytocrystallizetheseingly,thefieldisdevelopingintotwogroupings.largerassembliesandmembraneproteinsisincreas-Onegroupingarethosewhocontinuetoworkinglychallengingandin