hapsiteer高级操作培训

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1、HAPSITEER/SmartPlusTrainingHAPSITEER/SmartPlus培训AdvancedERIQ/PlusIQ高级的ERIQ/PlusIQCourseOutline课程大纲Overview综述InstrumentSetup仪器设置Tune调谐Troubleshooting故障查找AdvancedDataReview高级数据处理CreatingAMDISLibraries创建AMDIS库RecognizingCo-Elution识别共淘析ChromatogramOverlay色谱图迭加AccessingQuantitationReports查访定量报告

2、BasicMethodWriting基本方法编写MethodEditorOverview方法编写器概述SIMMethodsSIM方法MethodSequencing方法序列BlankSubtraction空样扣除Communications通讯InstrumentSetup仪器设置InsertGasCanisters插入气瓶TurnontheAnalyticalModuleortaketheAnalyticalModuleoutofExtendedStandby将分析模件电源on或将分析模件离开延伸的待机状态Systemwillheat,AutoTuneandrunaCon

3、centratorcleanout系统将加热,自动调谐和运行浓缩器清理TurnontheLaptop将计算机电源onDoubleclicktheERIQ/PlusIQicontoopenthesoftware双击ERIQ/PlusIQ图标,打开软件Tune调谐TuneOverview调谐概述AutoTune自动调谐ManualTune手动调谐TuneOverview调谐概述TuneoptimizestheperformanceoftheMS调谐使MS的性能最佳化AnimproperlytunedMScanresultin:未正确调谐的MS可导致:Lowsensitivity

4、灵敏度低Undetectedcompounds未能检测到化合物Massmisalignment质量数未对准Incorrectidentifications不正确的ID鉴别IncorrectMassRatios不正确的质量比LowNetFitorSInumbers低纯拟合或SI数TuneOverview调谐概述TenmassesareusedwhentuningtheMS调谐MS使用10个质量数Massesarebetween50-282toencompassthefull41-300rangeofmassfragments质量数在50-282之间包含整个41-300质量分裂

5、物范围TheInternalStandardgascontainsthechemicalsthatprovidethesemassesusedfortuning内部标准气体包含提供这些用于调谐质量数的化合物AutoTune自动调谐TheHAPSITEwillautomaticallyAutoTune:HAPSITE将自动调谐:UponStartup当启动时UponexitingExtendedStandby当退出延伸的待机状态时After12hoursofoperation运行12个小时后AutoTune自动调谐AutoTunecanbeinitializedby:自动调谐

6、可由下列动作起始:ClickingTune>>AutoTuneonthelaptop在计算机上单击Tune>>AutoTuneTouchingRUNAUTOTUNEonthefrontpanel触摸面板上的RUNAUTOTUNE按钮RebootingtheHAPSITE重新启动HAPSITETroubleshootingaFailedAutoTune不成功自动调谐的故障查找InternalStandardCanister内部标准气瓶Verifythatthepressureisadequate核实压强是否适当Verifytheexpirationdate核实是否已到使用期限

7、InsertnewIScanisterifnecessary必要时插入新的IS气瓶CarrierGas(SmartPlusOnly)载气(仅SmartPlus)Verifypressurethatthepressureisadequate核实压强是否适当Reservoirpressure(>400kPa)贮存器压强(>400仟帕)ERdoesnothaveareservoirER无贮存器InsertnewCarrierGascanisterifnecessary必要时插入新的载气瓶RepeatAutoTune重新执