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1、FungalGeneticsandBiology49(2012)271–283ContentslistsavailableatSciVerseScienceDirectFungalGeneticsandBiologyjournalhomepage:www.elsevier.com/locate/yfgbiTheGproteinbsubunitcontrolsvirulenceandmultiplegrowth-anddevelopment-relatedtraitsinVerticilliumdahliaea,
2、⇑aabAlikiK.Tzima,EpaminondasJ.Paplomatas,DimitriosI.Tsitsigiannis,SeogchanKangaLaboratoryofPlantPathology,AgriculturalUniversityofAthens,75IeraOdos,11855Athens,GreecebDepartmentofPlantPathology,ThePennsylvaniaStateUniversity,UniversityPark,PA16802,USAarticle
3、infoabstractArticlehistory:TogaininsightintotheroleofGprotein-mediatedsignalinginvirulenceanddevelopmentofthesoil-Received29April2011borne,wiltcausingfungusVerticilliumdahliae,theGproteinbsubunitgene(namedasVGB)wasdis-Accepted13February2012ruptedintomatorace
4、1strainofV.dahliae.Aresultingmutantstrain,70DGb15,displayeddrasticAvailableonline24February2012reductioninvirulence,increasedmicrosclerotiaformationandconidiation,anddecreasedethylenepro-ductioncomparedtothecorrespondingwildtype(wt)strain70wt-r1.Moreover,70D
5、Gb15exhibitedanKeywords:elongatedratherthanradialgrowthpatternonagarmedia.Atransformantof70DGb15(namedasSignaling70DGbPKAC1)thatcarriesanextracopyofVdPKAC1,aV.dahliaegeneencodingthecatalyticsubunitofcAMP-PKApathwaythecAMP-dependentproteinkinaseA,exhibitedwtg
6、rowthpatternandconidiation,wasunabletoformPathogenicitygeneWiltdiseasemicrosclerotia,producedhighamountsofethylene,andexhibitedvirulencebetweenthatof70DGb15and70wt-r1ontomatoplants.Phenotypicalchangesobservedin70DGb15and70DGbPKAC1correlatedwithtranscriptiona
7、lchangesinseveralgenesinvolvedinsignaling(MAPkinaseVMK1)anddevelopment(hydrophobinVDH1andACCsynthaseACS1)ofV.dahliae.ResultsfromthepresentworksuggestalinkagebetweenVGBandVdPKAC1signalingpathwaysinregulatingvirulence,hormoneproductionanddevelop-mentinV.dahlia
8、e.Ó2012ElsevierInc.Allrightsreserved.1.Introductionareformedandspreadsystemicallyviathexylemvessels,inwhichthefungusbecomesinaccessibletochemicalcontrolagentsVirulenceoffungalpathogensiscontroll
