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1、SnapShot:EndocyticTraffickingMarnixWieffer,1TanjaMaritzen,1andVolkerHaucke1,21FreieUniversitätandCharitéUniversityMedicineBerlin,2LeibnizInstituteofMolecularPharmacology,Berlin,Germany382Cell137,April17,2009©2009ElsevierInc.DOI10.1016/j.cell.2009.04.012Seeonlineversionfor
2、legendandreferences.SnapShot:EndocyticTraffickingMarnixWieffer,1TanjaMaritzen,1andVolkerHaucke1,21FreieUniversitätandCharitéUniversityMedicineBerlin,2LeibnizInstituteofMolecularPharmacology,Berlin,GermanyMechanismofClathrin-MediatedEndocytosisClathrin-mediatedendocytosisb
3、eginswithcoatnucleationmediatedbycargoandphosphatidylinositol(4,5)-bisphosphate,PI(4,5)P.Duringthisprocess,cargomol-2eculesareclusteredandconcentratedwithintheemergingcoatedpit.Aftercoatassemblyandmembraneinvagination,whichisfacilitatedbyBARdomain-containingproteins,vesic
4、lefissioniscatalyzedbytherecruitmentandhelicalassemblyoftheGTPasedynaminattheneckoftheburgeoningvesicle.MembranescissionrequiresthecooperativeactivityofdynaminwithSH3domain-containingproteins,manyofwhichpossessBARdomainsandregulatetheactincytoskeleton.Actinisrequiredforen
5、docytosisinyeastandmayhavearegulatoryroleinmammaliancells.Theactin-associatedmotorproteinsmyosin1EandmyosinVIarelinkedtotheendocyticmachineryviadynaminorsynaptojaninandDab2,respectively.Duringvesicleuncoating,primaryendocyticvesiclesmayacquireRab5,asmallGTPaseinvolvedintr
6、ansporttoearlyendosomesandinearlyendosomedockingandfusion.Primaryendocyticvesiclesaretargetedtoandfusewithearlyendosomesexpressingphosphatidylinositol(3)-phosphate,PI(3)P,inaprocessdependentonRab5effectorproteinsandearlyendosomalSNAREs.Differentialsortingofcargodestinedfo
7、rrecycling(requiringRab11andpossiblyRab4)orforlateendosome/lysosomaldegradation(requiringRab7)canoccuratthelevelofclathrin-coatedpitsandbecom-pletedinearlyendosomalsubdomains.Transportofcargofromearlyendosomestothetrans-Golginetworkrequirestheretromerproteincomplex.Pathwa
8、ysofEndocytosisEndocyticpathwayscanbegroupedaccordingtotheirrequirementfortheself-polymerizingve
