The contribution of pseudouridine to stabilities and structure of RNAs

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1、NucleicAcidsResearchAdvanceAccesspublishedDecember24,2013NucleicAcidsResearch,2013,1–10doi:10.1093/nar/gkt1330ThecontributionofpseudouridinetostabilitiesandstructureofRNAs1112ElzbietaKierzek,MagdalenaMalgowska,JolantaLisowiec,DouglasH.Turner,11,ZofiaGdanie

2、candRyszardKierzek*1InstituteofBioorganicChemistry,PolishAcademyofSciences,61-704Poznan,Noskowskiego12/14,Poland2andDepartmentofChemistry,UniversityofRochester,Rochester,NY14627,USAReceivedOctober14,2013;RevisedNovember23,2013;AcceptedNovember30,2013ABSTRA

3、CTribosewhichiscorrelatedwithanticonformationalpref-erencesofnucleobases(3).PublishedresultsdemonstrateThermodynamicdataarereportedrevealingthatstructuralrigidityofwithinsingle-anddouble-strandedDownloadedfrompseudouridine())canstabilizeRNAduplexesRNAregio

4、ns,butthebasictopologyofUandcontain-whenreplacingUandforming)-A,)-G,)-UandingRNAisusuallysimilar(1,3–10).InamodelfortheU2)-Cpairs.StabilizationisdependentontypeofsnRNAinvolvedinsplicingofpre-mRNA,however,basepair,positionof)withintheRNAduplex,-inducedsigni

5、ficantchangesofthearchitecturalland-andtypeandorientationofadjacentWatson–CrickscapeofthespliceosomalbranchsiteofRNAcomparedhttp://nar.oxfordjournals.org/pairs.NMRspectrademonstratethatforinternalwithanunmodifiedcounterpart(11,12).)-A,)-Gand)-Upairs,theN3imi

6、noprotonisisfoundintheTCloopofalmostalltRNAs.ForhydrogenbondedtotheoppositestrandnucleotidemosttRNAs,isalsofoundintheDstemand/orintheanticodonstemandloop(1).contributestostabilizationandtheN1iminoprotonmayalsobehydrogenofspecificstructuralmotifs.Forexample,

7、isphylogen-bonded.CDspectrashowthatgeneralA-helixeticallyconservedinmajorsplicesomalsnRNAs(U1,U2,structureispreserved,butthereissomeshiftingU4,U5andU6)andmostlylocatedinregionsinvolvedinofpeaksandchangingofintensities.)hastwoRNA–RNAandRNA–proteininteractio

8、nsessentialforhydrogendonors(N1andN3iminoprotons)andassemblyofthesplicesomeandforthesplicingprocessbyguestonDecember25,2013twohydrogenbondacceptorsbecausetheglyco-(12–14).The50endofU2snRNAbindsdirectlytopre-s