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1、Chemosphere93(2013)990–996ContentslistsavailableatSciVerseScienceDirectChemospherejournalhomepage:www.elsevier.com/locate/chemosphereToxicitytestingwithluminescentbacteria–CharacterizationofanautomatedmethodforthecombinedassessmentofacuteandchroniceffectsJ.Menz,M.Schneider,K.K
2、ümmerer⇑InstituteofSustainableandEnvironmentalChemistry,LeuphanaUniversityLüneburg,Scharnhorststraße1/C13,21335Lüneburg,GermanyhighlightsAnewmethodforanalysisofacuteandchroniceffectstowardsluminescentbacteria.Improvedsensitivitytowardssubstanceswithtime-dependenttoxicity.Go
3、odcorrelationtoconventionalmethodsandoutstandingreproducibilityofdata.Downscalingtomicrotitrationscaleincreasessamplethroughputandreducescosts.Methodisveryusefulasaninitialscreeningtestandisalreadyroutinelyapplied.articleinfoabstractArticlehistory:Theluminescentbacteriatesta
4、ccordingtoENISO11348isfrequentlyappliedin(eco)toxicitytestingandReceived5December2012isapplicableforahugevarietyofenvironmentalandindustrialsamples.AbigdisadvantageofthisReceivedinrevisedform23May2013methodistheveryshortexposuretime,whichisexpressedinalowsensitivityinregardtos
5、ubstancesAccepted25May2013withadelayedeffect.Chroniceffects,i.e.interferencewithcellgrowth,cannotbeassessedwiththiscon-Availableonline24June2013ventionalstandardmethod.ThegoalofthisresearchwastodevelopanautomatedtestingsystemforlongtermtoxicitytowardstheluminescentbacteriaVibr
6、iofischeribyimplementingmicrotitration-basedKeywords:instrumentation.Theoptimizedmethod,hereinafterreferredtoas‘‘kineticluminescentbacteriatest’’,Vibriofischericanbedescribedasaminiaturizedcombinationoftheconventionalshort-termluminescenceinhibitionLuminescenceinhibitionGrowthin
7、hibitiontestaccordingtoENISO11348andthePhotobacteriumphosphoreumgrowthinhibitiontest(DIN38412-Chronictoxicity37).Thevalidationprocedureincludedtheevaluationofsixreferencecompounds(3,4-Dichloroaniline,Ecotoxicity3,5-Dichlorophenol,Chloramphenicol,Streptomycinsulfate,Potassiumdi
8、chromate,Zincsulfateheptahy-Environmentalriskassessmentdrate)
