转录因子拟南芥激活因子1整合 海藻糖代谢产物[Copy]对碳饥饿反应的影响

《转录因子拟南芥激活因子1整合 海藻糖代谢产物[Copy]对碳饥饿反应的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、TranscriptionFactorArabidopsisActivatingFactor1Integrates1[OPEN]CarbonStarvationResponseswithTrehaloseMetabolismPrashanthGarapati,ReginaFeil,JohnEdwardLunn,PatrickVanDijck,SalmaBalazadeh*,andBerndMueller-Roeber*InstituteofBiochemistryandBiology,UniversityofPotsdam

2、,14476Potsdam-Golm,Germany(P.G.,S.B.,B.M.-R.);PlantSignalingGroup(P.G.,S.B.,B.M.-R.)andSystemRegulationGroup(R.F.,J.E.L.),MaxPlanckInstituteofMolecularPlantPhysiology,14476Potsdam-Golm,Germany;andDepartmentofMolecularMicrobiology,VIBandLaboratoryofMolecularCellBio

3、logy,KULeuven,B–3001Leuven,Belgium(P.V.D.)ORCIDID:0000-0002-1542-897X(P.V.D.);0000-0002-5789-4071(S.B.);0000-0002-1410-464X(B.M.-R.).Plantsrespondtolowcarbonsupplybymassivereprogrammingofthetranscriptomeandmetabolome.Weshowherethatthecarbonstarvation-inducedNAC(fo

4、rNOAPICALMERISTEM/ARABIDOPSISTRANSCRIPTIONACTIVATIONFACTOR/CUP-SHAPEDCOTYLEDON)transcriptionfactorArabidopsis(Arabidopsisthaliana)TranscriptionActivationFactor1(ATAF1)playsanimportantroleinthisphysiologicalprocess.WeidentifiedTREHALASE1,theonlytrehalase-encodinggen

5、einArabidopsis,asadirectdownstreamtargetofATAF1.OverexpressionofATAF1activatesTREHALASE1expressionandleadstoreducedtrehalose-6-phosphatelevelsandasugarstarvationmetabolome.Inaccordancewithchangesinexpressionofstarchbiosynthesis-andbreakdown-relatedgenes,starchleve

6、lsaregenerallyreducedinATAF1overexpressorsbutelevatedinataf1knockoutplants.Attheglobaltranscriptomelevel,genesaffectedbyATAF1arebroadlyassociatedwithenergyandcarbonstarvationresponses.Furthermore,transcriptionalresponsestriggeredbyATAF1largelyoverlapwithexpression

7、patternsobservedinplantsstarvedforcarbonorenergysupply.Collectively,ourdatahighlighttheexistenceofapositivelyactingfeedforwardloopbetweenATAF1expression,whichisinducedbycarbonstarvation,andthedepletionofcellularcarbon/energypoolsthatistriggeredbythetranscriptional

8、regulationofdownstreamgeneregulatorynetworksbyATAF1.Trehaloseisanonreducingdisaccharideformedbyangenesencodeactiveenzymesconferringspatiotemporala,a-1,1