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1、antagomiragomirMolecularCancer.2011MinfengShu,etal.Hepatology.2010LinHuiLiang,NaturecellbiologyJieDing,etal.Science.2009etal.BonauerA,JClinInvest.2009LiH,XieH,JAshuthoshDharap,PLoSONENeriMercatelli,Nature.2008etal.ThomasThum,PLoSONELauraFontana,etal.NatureMedicine.2007Alessa
2、ndraCare,NucleicAcidsResearchJanKrützfeldt,etal.Nature.2005KrützfeldtJ,etal.CancerCell.2011JinHou,etal.JBC.2011DongLi,etal.PLoSONEMin-JieNi,etal..CerebmiRNA产品使用说明RN：R10034.3.BloodFlowMetabetal.2008.2008.2011etal.Reference.etal.etaletal..2010产品简介..2007TM.micrONmiRNAmimic是miRN
3、A模拟物，化学合成的成熟miRNA双链，即用型；2009TMmicrOFFmiRNAinhibitor是miRNA抑制物，化学修饰的成熟miRNA互补单链，即用型；miR-199a/b-3pnudemiceTMmil-HongrES2micrONmiRNAagomir是特殊化学修饰的miRNA激动剂，适用于细胞实验、动物实验，即用型；miR-335miR-125bmiR-151-5pnudemicetransplantionmiR-2861miR-222miR-221miR-17-5pmiR-133miR-192miR-122miR-99anu
4、demicemiR-92micemiR-145ratmiR-21miR-16miRNAAnimalTMmicrOFFmiRNAantagomir是特殊化学修饰的miRNA拮抗剂，适用于细胞实验、动物实验，即用型。nudemice4-week-oldnudemice6~8weeksoldfemalemice6-week-old6-week-oldmicemice10~12weeksoldnudemice6-week-oldmice8-week-oldmice6-week-oldmice6-week-oldSDrat7~10days运输保存产品以冻
5、干粉的形式，常温运输。收到产品后，请于-20℃~-80℃保存，冻干粉可以稳定保存一年。使用前瞬时离心，用RNase-freeH2O或灭菌ddH2O配制成20μM储存液，分装保存，避免反复冻融（不超过5次）。表4动物实验agomir和antagomir用量参考injectionIntratumorallyinjectionsubcutaneouslyintravenousinjectiontailveininjectionimplantationminipumpinjectionintratumorallyjugularveincatheteri
6、njectionintratumorallyimplantationminipumpinjectionIntracerebraltail-veininjectionsinjectiontumormassinjectiontumormassinjectioncaudaepididymis表120μM储存液的配置参考DeliverymiRNA(nmol)0.250.512520溶解体积(μl)12.525501002501000注：如需进行高内涵筛选试验，可选择miRNALibrary。4mg/mlbodyweight8mgperkgofweigh
7、t80mg/kgbodyeachrattotally960pmolantagomir1ugofeachweight80mgperkgbody0.2mgpermouseweight80mgperkgbody0.1ml~800ngpermouse0.2mlperinjectionasingleinjectionweight80mgperkgbody10nmol72hafterinjection10nmol4nmolperside使用前须知2x102x10Doses为避免外界因素（包括酶，极端pH或者温度条件等）导致产品降解，所有操作请严格遵循RNA
8、操作规则。实验过cellstransfectedbyantagomir5N/Acellstransfectedbyantagomir6N/A程中，产品
