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1、ApplMicrobiolBiotechnol(1986)24:180--185AppliedMicrobiologyBiotechnology©Springer-Verlag1986Screeningofwhite-rotfungiforbiologicalpretreatmentofwheatstrawforbiogasproductionH.W.MiillerandW.TrfischFraunhofer-InstitutfiirGrenzfl/ichen-undBioverfahrenstechnik,Nobelstr.12,D-7000Stuttgart80,FederalRepubl

2、icofGermanySummary.Twentytwobasidiomycetes,mostlywhiterotfungi,weregrownonwheatstraw.Lig-nin-,cellulose-,andhemicellulose-degradationwasrecordedinordertofindaspeciesgrowingonligninpreferably.The"oyster-mushroom"Pleurotussp."florida'"showedfastestdelignifica-tionofalltestedfungi.Strawpretreatedbythis

3、funguswasfermentedanaerobicallytobiogas.Thegasyieldproducedfrom"myco-straw"istwicetheamountfromun-treatedstraw.IntroductionLignocellulosebiomassiscontinuouslyproducedingreatquantities,givingrise,untilrecently,onlytodisposalproblems.Becauseofitschemicalcompositionitcouldbeanexcellentsubstrateforbiote

4、chnologicalprocesses.However,cellulosefi-brilsareimbeddedintoaligninmatrixwhichpro-tectsthemfromenzymaticattack.Straw,alignocellulosewastematerial,canbepretreatedchemicallyorphysicallytoincreaseitsbiotechnologicalsubstratevalue(Chahaletal.1981;Detroyetal.1981;Fanetal.1981).Whiterotfungicanbeusedfort

5、hebiologicalpretreatment.Theyaretheonlymicroorganismsknownfortheirabilitytodegradelignincom-pletely.Therefore,itshouldbepossibletousethesemicroorganismstodegradethelignincom-ponentinligno-cellulosicwastematerialtomakethecelluloseandhemicellulosecomponentsmoreaccessibleforfurtherbiotechnologicaluse.S

6、omeofthesefungiproduceediblefruitingbodies--aOffprintrequeststo:H.W.MOilerfact,thatmightbeimportantwhencalculatingprocesseconomy.Themostinvestigatedfungusforligninde-gradationisPhanerochaetechrysosporium(Keyseretal.1978;Kirketal.1978;Leisolaetal.1983;TienandKirk1983;Ulmeretal.1983)Inthepresentworkot

7、herwhiterotfungiweresearchedforselectiveligninremoval.Theaimofthisinves-tigationistousethe"myco-straw"asasubstrateinafurtherbiotechnologicalprocess:biogaspro-duction.Previousinvestigationshavemainlybe