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1、APPLIEDANDENVIRONMENTALMICROBIOLOGY,Aug.2006,p.56485652Vol.72,No.80099-2240/06/$08.000doi:10.1128/AEM.00727-06Copyright©2006,AmericanSocietyforMicrobiology.AllRightsReserved.CarbonIsotopeFractionationduringAcetoclasticMethanogenesisbyMethanosaetaconciliiinCultureandaLakeSediment1121HolgerPe
2、nning,PeterClaus,PeterCasper,andRalfConrad*1MaxPlanckInstituteforTerrestrialMicrobiology,Karl-von-FrischStr.,35043Marburg,Germany,andLeibnizInstituteof2FreshwaterEcologyandInlandFisheries,DepartmentofLimnologyofStratifiedLakes,16775Stechlin,GermanyReceived29March2006/Accepted31May2006Theisoto
3、peenrichmentfactors()inMethanosaetaconciliiandinalakesediment,whereacetatewasconsumedonlybyMethanosaetaspp.,wereclearlylessnegativethantheusuallyobservedforMethanosarcinaspp.Thefractionofmethaneproducedfromacetateinthesediment,asdeterminedbyusingstableisotopesignatures,was10to15%lowerwhent
4、heappropriateofMethanosaetaspp.wasused.Methaneisamajorproductofthedegradationoforganicwasaddedtoaninitialconcentrationofapproximately70mM.13matterinanoxicenvironmentslikericepaddies,naturalForthetotalmoleculetheaddedacetate(ac)hadaCacof13wetlands,andlakesediments.ItsproductionfromH2-CO224
5、.0,andforthemethylgrouptheCac-methylwasandacetate(ac)innaturalsystemshasbeenquantifiedby29.4.Afterinoculationwitha20%bacterialsuspensioninstableisotopemodeling(7,17).Oneoftheinputparametersthelateexponentialphase(resultinginafinalvolumeof125neededisthecarbonisotopefractionationofacetoclastic
6、ml),severalgas(0.4-ml)andliquid(2-ml)sampleswerere-methanogenesis.Todeterminethefractionationfactor()movedandusedforanalysisofpH,concentration,andthe13incultureorinanenvironmentalsystem,theCofthecarbonisotopecompositionofacetateandtheproductsmethylcarbonofacetate(ac-methyl)mustbeknown,sinc
7、eformed.CH4isproducedfromthemethylcarbonratherthantheSedimentsamplesfromeutrophicLakeDagow(Northerncarboxylcarbonofacetate(5,19).ForMethanosarcinaspp.Brandenburg,Germany)wereobtainedon9November2004isotopefractionationhasbeendeterminedinpureculturea
