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1、第39卷第2期Vol.39No.2第22016期年2月EnvironmentalScience&TechnologyFeb.2016李林,陈峰,赵荣芳.鄱阳湖灰化苔草浸泡液对铜绿微囊藻的化感作用[J].环境科学与技术,2016,39(2):1-5.LiLin,ChenFeng,ZhaoRongfang.AllelopathiceffectofsoakingCarexcinerascensinPoyangLakeonMicrocystisaeruginosa[J].EnvironmentalScience&Technology,2016,39(2):1-5.鄱阳湖灰化苔草
2、浸泡液对铜绿微囊藻的化感作用121李林,陈峰,赵荣芳(1.江西师范大学鄱阳湖湿地与流域研究教育部重点实验室,江西南昌330022;2.江西省水利规划设计院,江西南昌330029)摘要:在室内控温、控光条件下,研究鄱阳湖洲滩优势植物灰化苔草浸泡液对铜绿微囊藻生长的影响。试验得到,灰化苔草浸泡液对铜绿微囊藻的抑制作用较高;枯灰化苔草浸泡液对铜绿微囊藻的抑制率在81%~88%,随浸泡液植物生物量含量的增加而增加,当浸泡液植物生物量含量大于24g/L后对铜绿微囊藻抑制率差异较小;鲜灰化苔草浸泡液对铜绿微囊藻的抑制率大于90%,浸泡液植物生物量含量超过6g/L时,对铜绿微囊藻抑制
3、率差异较小;灰化苔草浸泡液中化感物质使微囊藻细胞直径较对照组减小8%~12%,藻细胞发生固缩现象;微囊藻细胞超氧化物歧化酶(SOD)活性在0.57~1.14U/106cell之间占对照组的30%~60%,藻细胞内氧化活性降低,胞内过氧化氢物不能及时去除在胞内积累,单位藻细胞丙二醛(MDA)增加3~4倍,藻细胞膜脂过氧化使藻细胞生长受到抑制。实验结果得到鄱阳湖洲滩优势植物灰化苔草在夏季将对蓝藻水华产生抑制作用,这为开发和利用鄱阳湖岸滩优势植物苔草生态控藻提供基础实验数据。关键词:灰化苔草;铜绿微囊藻;浸泡液;化感抑藻中图分类号:X172文献标志码:Adoi:10.3969
4、/j.issn.1003-6504.2016.02.001文章编号:1003-6504(2016)02-0001-05AllelopathicEffectofSoakingCarexcinerascensinPoyangLakeonMicrocystisaeruginosa121LILin,CHENFeng,ZHAORongfang(1.KeyLaboratoryofPoyangLakeWetlandandWatershedResearchofMinistryofEducation,JiangxiNormalUniversity,Nanchang330022,China
5、;2.JiangxiProvincialWaterConservancyPlanningandDesignInstitute,Nanchang330029,China)Abstract:TheinhibitioneffectsofsoakingliquorCarexcinerascens,whichwasdominantplantsinPoyangLakeriverbeach,onMicrocystisaeruginosawerestudied,undercontrollingtemperature,lightandnutrientsterileconditions.T
6、heresultsshowedthatgrowthofMicrocystisaeruginosaweresignificantlyinhibitedinCarexcinerascenssoakingculture,withtheinhibitionrateintherangeof81%~88%,andincreasedwithbiomassandnotsignificantlydifferentwhenplantbiomasswasabove24g/LindeadCarexcinerascensculture;inhibitionratewasabove90%andwa
7、snotsignificantlydifferentinfreshCarexcinerascenssoakingculturewhenplantbiomasswasabove6g/L.Microcystisaeruginosacelldiametersintreatmentgroupscutdown8%~12%thancontrolgroup,thealgalcellproducedthepyknosis.Superoxidedismutase(SOD)activitybetween0.57U/106celland1.14U/106cel