药学本科_毕业论文正稿

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1、Word格式沈阳药科大学本科毕业论文论文题目：化合物A联合化合物B对人肝癌HepG2细胞作用机制研究起止时间：2014年10月26日—2015年6月20日姓名学号：学院专业：年级班级：指导教师：XXX副教授实习单位：XXX大学生命科学与生物制药学院药理系完美整理Word格式完美整理Word格式完美整理Word格式目录中文摘要······································································1英文摘要·································

2、·····································2第1章绪论··································································31.1细胞凋亡·····························································31.2立题依据·····························································5第2章实验部分················

3、··············································62.1实验材料·····························································62.2实验方法·····························································72.3实验结果····························································12第3章结论与讨论·········

4、··················································173.1完美整理Word格式结论································································173.2讨论································································18参考文献·······························································

5、······21致谢·········································································23完美整理Word格式摘要原发性肝癌是世界常见的恶性肿瘤之一，也是我国的高发恶性肿瘤，由于不易被早期诊断，进展迅速，术后容易复发、转移，因此预后较差。目前肝癌仍以外科手术为公认首选和主要的治疗手段，但由于很多肝癌患者确诊时已是中晚期，失去了手术治疗的机会并于诊断后1年内死亡，手术切除率<30%，术后总体5年生存率仅30%~40%。对于可手术的肝癌患者，术后复发、转移是影响

6、治疗效果及预后的主要因素，对于不能手术的中晚期肝癌患者尚缺乏有效的治疗方法。传统的化疗、放疗对肝癌疗效欠佳，目前仍缺乏行之有效的化学预防和治疗靶位[1]。因而本课题是对化合物A联合化合物B对人肝癌HepG2细胞作用机制研究。本实验首先用MTT法分别测得不同浓度的A和B单独处理HepG2细胞及不同浓度的A和B联合用药处理HepG2细胞死亡的影响。结果表明完美整理Word格式不同浓度的A和B单独处理HepG2细胞，增值抑制程度表现为时间剂量依赖效应，并且在A6μmol/L联合B100μmol/L协同作用最为显著。流式细胞仪检测细胞凋亡率显示

7、，联合用药组凋亡率升高更为明显。WesternBlot法检测蛋白显示，促凋亡相关蛋白Bax、FADD增加，抗凋亡蛋白Bcl-2表达减少。关键词联合用药，细胞凋亡，作用机制完美整理Word格式AbstractHepatocellularcarcinoma(HCC)isthemostcommoncancerintheworldandanincreasedmalignantillnessinourcountry.Becauseofitsaggressivebiologicalbehavior,rapidlyprogressesanddelay

8、eddiagnosis,thismalignancyhasagrimprognosisevenfollowingsurgicalresectionandhashighdisease-recurrence.S