资源描述：
《生长、活性和温度响应 氨氧化古细菌与细菌 土壤微观结构》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、EnvironmentalMicrobiology(2008)10(5),1357–1364doi:10.1111/j.1462-2920.2007.01563.xGrowth,activityandtemperatureresponsesofammonia-oxidizingarchaeaandbacteriainsoilmicrocosmsMariaTourna,ThomasE.Freitag,GraemeW.NicolIntroductionandJamesI.Prosser*Molecular
2、techniqueshavedemonstratedthatArchaea,SchoolofBiologicalSciences,UniversityofAberdeen,traditionallyassociatedwithextremeenvironments,con-CruickshankBuilding,St.MacharDrive,AberdeenAB24stituteasignificantproportionofprokaryoticcellabun-3UU,UK.danceinnon-t
3、hermophilicenvironments.Inparticular,‘Group1’crenarchaea(DeLong,1998)representsignifi-Summarycantproportionsofprokaryotesinmarineplankton(e.g.Karneretal.,2001;Herndletal.,2005)and1%ofAmmoniaoxidation,asthefirststepinthenitrifica-soilprokaryotes(Sandaaetal
4、.,1999;Ochsenreiteretal.,tionprocess,playsacentralroleintheglobalcycling2003).Theabundanceofcrenarchaeaimpliestheirimpor-ofnitrogen.Althoughbacteriaaretraditionallycon-tanceinbiogeochemicalcyclesandecosystempro-sideredtoberesponsibleforammoniaoxidation,
5、acesses,whilethedifferentphysiologicalcharacteristicsofroleforarchaeahasbeensuggestedbydatafromarchaeaandbacteriaprovidethepotentialforsignificantmetagenomicstudiesandbytheisolationofamarine,differencesinprocesscharacteristicsandratesfromautotrophic,ammo
6、nia-oxidizing,non-thermophilicthoseofalreadycultivatedgroups.crenarchaeon.EvidenceforammoniaoxidationbyRecentmetagenomicstudies(Schleperetal.,2005;non-thermophiliccrenarchaeainmarineandterres-Treuschetal.,2005;Hallametal.,2006)havedemon-trialenvironment
7、sislargelybasedonabundanceofstratedco-occurrenceofcrenarchaeal16SrRNAgenesbacterialandarchaealammoniamonooxygenaseandgeneshomologoustothoseencodingbacterial(amo)genes,ratherthanactivity.Inthisstudy,weammoniamonooxygenase,AMO,akeyfunctionalhavedetermined
8、theinfluenceoftemperatureontheenzymeinammoniaoxidation,thefirststepinnitrifica-responseofammonia-oxidizingbacteriaandarchaeation.CrenarchaealamoAgenesappeartobeubiqui-innitrifyingsoilmicrocosmsusingtwoapproaches,touslydistributedins
