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1、2010年23卷3期西南农业学报Vol123No13SouthwestChinaJournalofAgriculturalSciences801文章编号:1001-4829(2010)03-0801-04普洱茶生茶抗疲劳作用研究*黄业伟,邵宛芳,冷丽影,张冬英(云南农业大学龙润普洱茶学院,云南昆明650201)摘要:研究普洱茶生茶对小鼠的抗疲劳作用,为普洱茶保健功效研究提供一定的理论依据。将80只昆明种小鼠(雌雄各半,体重25?4g)随机分为4组(20只/组),即空白对照组和普洱茶生茶高、中、低剂量组,分别灌胃0.9%生理盐水
2、和2.0g/kg.bw、1.0g/kg.bw、0.5g/kg.bw的普洱茶生茶。按照小鼠体重给予不同体积的受试物,自由摄食与饮水,连续灌胃30d,测定小鼠力竭游泳时间。采血、分离血清并取组织样,测定血乳酸(BLA)、血尿素氮(BUN)、血乳酸脱氢酶(LDH)及肝糖元(LG)、肌糖元(MG)指标。结果显示:与空白对照组相比,普洱茶生茶中、低剂量组显著延长了小鼠游泳时间(P<0.01),尤以中剂量组作用明显,增加率达50.68%;显著降低了运动后血乳酸(P<0.01)和血尿素氮(P<0.05)水平,提高了血清乳酸脱氢酶活力(P<0
3、.01),肌糖元和肝糖元的储备量的增加达极显著水平(P<0.01)。因此,普洱茶生茶具有良好的抗疲劳生理活性,以中剂量组作用最明显。关键词:普洱茶生茶;抗疲劳中图分类号:S571.1文献标识码:AResearchofAnt-ifatigueEffectofUnfermentedPu-erhTeaHUANGYe-we,iSHAOWan-fang,LENGL-iying,ZHANGDong-ying*(CollegeofLongrunPu-erhTea,YunnanAgriculturalUniversity,YunnanKunm
4、ing650201,China)Abstract:Theant-ifatigueeffectofunfermentedPu-erhteaonmicewasstudiedtoprovidesometheoriesonthefunctionarymechanismofun-fermentedPu-erhtea.80micewererandomlyassignedtoblankcontrolgroup,highdose,middledoseandlowdoseexperimentalgroupsac-cordingtotheirbo
5、dyweights.Normalsalineof0.9%wasadministeredinblankcontrolgroup.UnfermentedPu-erhteawasadministeredinexperimentalgroupswithdosesof2.0,1.0,0.5g/kg.bw,respectively.Eachgroupwasgivensamefoodandwater.After30days,theswimmingtestwerecarriedoutandthedeathtimewasobservedandr
6、ecorded.Afterthemiceswimmingforaperiod,themicebloodwastakenfordeterminingbloodlactic-acid,bloodureanitrogen(BUN)andlactatedehydrogenase.Theliverandmusclewastakenfordeterm-iningliverandmuscleglycogen.Theresultsshowedasfollows:comparedwithblankcontrolgroup,Unfermented
7、Pu-erhteaofmiddledoseandlowdoseexperimentalgroupscansignificantlyprolongswimmingtime(P<0.01),reducelactic-acid(P<0.01)andbloodureanitrogenlevel(P<0.05),increaselactatedehydrogenaselevel(P<0.01)andincreaseliverglycogenandmuscleglycogenstorage(P<0.01)afterswimming.So,
8、UnfermentedPu-erhteacanalleviatephysicalfatigueofmice,andthemiddledoseexperimentalgroupshadsignificantant-ifatigueeffect.Keywords:Unfermen
