用原子力显微镜分析细胞弹性变化对整合素与配体相互作用的影响

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1、重庆大学硕士学位论文英文摘要AbstractThecellelasticpropertyisoneimportantparameteronthecellbiomechanicsresearch,thestructureandphysiologicalfunctionsofcellsarecloselyrelatedwiththegrowthenvironmentofcell,andthephysicalchangingofmicroenvironmentofcellcannotonlycausethecellelasticitychangedbutalsot

2、heexpressionofintegrinandoutsideofthecellorientation.Theycanaffecttheinteractionsbetweenintegrinandtheirligands.Thechitosan/polyacrylamidehydrogelisavariablesubstratematerial,andHepG2cellsareplantedondifferentrigidityofchitosan/polyacrylamidehydrogensubstrates.After48hours,morpholo

3、gicalcharacteristicsofHepG2cell(suchasspreadarea,long/shortaxisratio)aremeasured,andthecytoskeletonisobservedbyFITC-phalloidin,thentheexpressionofintegrinα6β1isdetectedusingFCM;thesurfacetopography;cellelasticityofHepG2indifferentrigiditysubstrateandtheinteractionsbetweenintegrinα6

4、β1andLNunderphysiologicalconditionsaredetectedbyAFM.Themainresultsobtainedinthisstudyasfollows:(1)Therigidityofchitosan/polyacrylamidehydrogelisadjustable,andcanbeusedasbasematerialforcellmechanicsresearch.(2)Thegreaterbasalrigidity,themorespreadareaandlong/shortaxisratioofHepG2cel

5、l.(3)ThesurfacetopographyandcellelasticityofHepG2cellarediscrepantindifferentrigidity.Thelesspseudopodiaextendingfromcelledgeinthehardnessofsubstrateof1000Pa,themorepseudopodiainthecontrolgroup.TheYoung’smodulusofHepG2cellsinthecontrolgroup,3500Paand1000Parigiditysubstrateare2036+1

6、045Pa,714+311Paand803+445Pa.(4)Theadhesionproportionofcellisdifferentineachrigidity,theinteractionforcebetweenintegrinα6β1andligand(LN)are19±7pN、37.5±15.8pNand38.85±19.7pN.(5)ThecytoskeletoncolorationsdetectedthatthecytoskeletonofHepG2isclearandthroughoutthecellincontrolgroup,while

7、itisnotobviousanddistributesintheedgeofcellsinexperimentalgroup.(6)Theexpressionofintegrinoncellsurfaceindifferentrigidity:theintegrinα6β1increaseswhensubstratebecomessofter(P<0.05).Keywords:Atomicforcemicroscope;Cellelasticity;Receptor/Ligandinteraction;Integrinα6β1II重庆大学硕士学位论文目录目

8、录中文摘要.....................