盐胁迫对小麦代换系幼苗的影响与染色体效应分析

盐胁迫对小麦代换系幼苗的影响与染色体效应分析

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时间:2019-06-25

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1、万方数据6D、CS、Synthetic6x耐盐性较弱。关键词:小麦代换系:盐胁迫;染色体效应;生理指标;幼苗万方数据PhysiologicalandChromosomalEffectsofSaltStressonChromosomeSubstitutionofWheatSeedlingsAuthor:JingJiao-jiaoMajor.BotanySupervisor:ProfessorBaiZhi·)ringAbstractSoilsalinizationisoneofthemostimportantfactsinfluencinggloba

2、lagricultureandcropproduction.Asweknowwheatisanimportantcropintheworld,whichismoderatelysalt-tolerantandtheyieldofwheatispositivelysignificantcorrelatedwiththesaltcontent.Inordertofulfilltheneedofworld’Sgrowingpopulationforfood,itbecomestheresearchfocusofdomesticandforeignexp

3、ertstostudytheeffectofwheatsalttoleranceandtobreedhigh-resistedspecies.Thewheatsubstitutionlinesarethepreciousgermplasmresourcesofgeneticresearch.ChinaSpring(cs)-Synthetic6xchromosomesubstitutionlinesaregeneratedbythe21chromosomeofdonorvarietiesSynthetic6xintotherecipientvari

4、etyChineseSpring.TherearegreatgeneticdifferencesbetweenmaleSynthetic6x,andfemaleChineseSpringbecauseofmaleSynthetic6xcontainingwealthyresistancegeneandrichgeneticdiversity.Inordertostudytheeffectandchromosomalcontrolofsaltstressinwheatseedlings,wheatsubstitutionlinesbetweenCh

5、ineseSpringandSynthetic6xwereculturedinHoagland’Ssolution.Twotreatmentsincludingcontrolgroup(0mmolL叫NaCl)andsalttreatmentgroup(150mmol’L叫NaCI)werecarriedoutattwo-leafstageofseeding..Physiologicalandbiochemicalindexesweremeasuredundercontrolledconditionandsalttreatmentofseedli

6、ngsatfour-leafstage.Itwouldprovideatheoreticalbasisforthebreedingandgeneticimprovementofsalt-tolerantgenotypes.Theresultswereasfollowing:1.Theresultsshowedthatsaltstessresultinaccumulationofsolubleproteincontentandsolublesugarcontent,prolinecontent,Na+,K十content,SOD,PODactivi

7、tiesandreductionofspecificleafweigh,chlorophyllcontent,carotenoidcontent,Photosyntheticrate,transpirationrate,MDAcontent.Thegenesincreasimgsolubleproteincontentmightbeexiston1A,2A,4A,6Achromosomesandthegenespromotingsolublesugarcontentmightbelocatedon6A,7A,7Dchromosomeaswella

8、sthegenespromotingprolinecontentmightbelocatedon7A,6DchromosomeofSyn

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