地西泮减弱5-羟色胺诱导的大鼠离体胸主动脉环收缩作用与其机制

《地西泮减弱5-羟色胺诱导的大鼠离体胸主动脉环收缩作用与其机制》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、山西医科大学硕士学位论文Diazepamattenuates5一hydroxytryptamine—inducedcontractionsinadultandoldratsthoracicaortaABSTRACTobjective：’I’0observeandcomparetheeHbctsofDZPonthecontractioninducedby5-hydrox”rypt锄ine(5一HT)inisolatedthoracicaorta“ngsofadultandoldrats；toexploremee疏ctsofdrugsonthef

2、．oregoingphenomellaandtheirpossiblemechanisms．Methods：ThemethodofisolatedVasculartensionrecordsyst锄wasfollowed．TheadultandoldratsweresacrificedbycerVicalVertebra1uxationandthethoracicaortawasquicklyisolated仔omeach锄imalandplacedincoldphysiolo西calsaltsolution(PSS)，th饥thesun．o

3、undingco衄ectivetissuesandfatwerecare如llyremoVed．TheVesselwascutinto—ngsabout3—4mminleIl垂h．TWostainless—steelwireswerepassedthroughthel啪enofeachring．Onewasfixedtothebottomofthebathch锄ber；theotherwasconnectedtoanisometricforcetransducer(BL一420Fsyst锄)tomeasurethetensionoftheri

4、ng．Ringswereplacedina10mlch锄bercontainingPSS(PH=7．4)，bubbledwith100％02，at37℃．Thedngswerestretcheduntilmeyexenedanoptimalbasaltensionof29，aIldthenwereallowedtoequilibrateforlhourwiththebathnuidbeingchangedeVe巧15minutes．Ef．fectsofDZPonthecontra“onsinducedby5-HTinmethoracicaon

5、icringswithintactendotheliuIn0rdenudedendotheli啪wereobserVed．TheefjE．ectsofVadousdrugsincludeⅣ：-nitro也．ar百ninemethylester(L—NAME，1O．4mol／L)，indomethacin(Indo，10。5mol／L)andPK11l95(1O。omol／L)，tetraethyl锄moni啪(TEA’10屯mol／L)，4一锄inop)，ridine(4一AP，l0。3mol／L)，舀ibenclaIIlide(Gli，10

6、‘5mol／L)，bariumchlonde(BaCl2，lO。3mol／L)werealsostlldied．Results：(1)5一HT(10～·3x10。mol／L)couldproducetheconcentration—d印endentcontractionsinisolatedthoracicaortaringsofadultandoldrats．TheEC50of5-HTw勰(3．4l士0．18)×10．6mol／Lor(1．96士0．12)×10_6mol／Linintactordelludede11domeliumisol

7、atedthoracicaortaringsofadultratsrespectiVely．TheEC500f5-HTwas(2．92士O．15)×10．6mol／Lor(3．15士O．15)×lO-6mol／LinintaCtordenudedendotheliumisolatedmoracicaortaringsofoldratsrespeCtiVely．(2)ontheadultandoldratsisolatedthoracicaortaringswi廿lintact0rdenuded饥dotheli啪，DZP(3×lO‘6mol／L

8、，10-4mol／L)attenuatedthe5一HT．inducedvasoconstriCtion；DZP(3×10．6m01／L，1O-4mol／L)dec