蛋白质在纳米材料2f离子液体复合膜修饰电极上直接电化学与电催化

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1、DIRECTELECTRoCHEMISTRYANDELECTRoCATA“SIS0FPROTElNINNANOPARTICLE-IONICLIQUIDCOMPOSITEFILMMoDIFIEDELECTRODEABSTRACTNanomaterialshavemanyspecificphysical，electronicandchemicalcharacteristicssuchashighsurfacearea,goodbiocompatibilityandstrongadsorptionablity,whichhavebeenwidelyusedinthefieldofelectroch

2、emistry．Ionicliquids(ILs)havemanyuniquephysicochemicalcharacteristicssuchasrelativelyhighionicconductivity,goodchemicalandthermalstability,negligiblevaporpressure，wideelectrochemicalwindowsandgoodbiocompatibility,whichhadbeenusedintheareaofelectrochemistryandelectroanalysis．InthispapernoVelnanobioc

3、ompositematerialsWerefabricatedbymixingnanomaterialwithionicliquidandNationtogether,whichWasusedtoimmobilizetheproteinonthesurfaceofcarbonelectrode．Theelectrochemicalbehavioroftheproteinanditselectrocatalyticpropertieswca'estudied．ThemaincontentsofthisPaperareasfollows：(1)AnovelnanobiocompositeWasp

4、reparedbymixingZr02nannoparticle谢thionicliquid1-ethyl-3-methylimidazoliumtetrafluoroborate([EMIM]BF4)andNationtogether,whichWasfurtherusedtoimmobilizemyoglobin(Mb)onthesurfaceofcarbonionicliquidelectrode(CILE)．SpectroscopicresultsindicatedthatMbremaineditsnativestructureinthenanobiocompositemateria

5、l．Cyclicvoltammetricresultsshowedapairofwell—definedredoxpeaksappearedinpH7．0phosphatebuffersolution(PBS)，indicatingthedirectelectrontransferofMbWasrealizedonthemodifiedelecctrode．Mbexhibitedasurface—controlledelectrochemicalprocessandtheelectrochemicalparameterssuchaselectrontransfercoe伍cient(a)an

6、delectrontransferrateconstant僻)Werec．alculatedas0．499and0．813S～，respectively．ThefabricatedMbelectrodeshowedgoodeleetrocatalyticabilitytothereductionoftrichloroaceticacid(TCA)．Ⅱ(2)Ananobiocompatiblecompositenanoparticles(nano—ZnO)andioniccontaininghemoglobin(Hb)，ZnOliquid1-butyl··3··mcthylimidazoliu

7、mhexatiuorophosphate(BMIMPF6)wasfabricatedandfurthermodifiedontheglassycarbonelectrode(OCE)．TheeleetrochemicalbehaviorsofHbinthecompositefilmworecarefullystudiedandapairofquasi—reversibleredoxpeaksappearedi