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ID:39116295
大小:2.25 MB
页数:58页
时间:2019-06-25
《成纤维细胞与完全脱细胞人羊膜的三维黏附》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、大津医科人学硕十学位论文焦现象。HFF种植在TAHAM上8~14h后细胞移动速度可达12t.tm/h,当细胞在原位形成细胞团块时移动速度接近0I_tm/h。在TAHAM上细胞的移动轨迹接近直线,而在普通培养皿上的细胞移动为无序随意运动,移动速度为16~481.tm/h。结论:TAHAM保留了羊膜的原有主要成分和力学特征,HFF可在TAHAM上形成3D黏附,其细胞形态及细胞移动方式符合3D黏附特征。关键词:羊膜细胞黏附整合素类成纤维细胞3D黏附ll天津医科人学硕+学位论文Abstractobjective:Paxillinanda5integrincoloc
2、alizedtocell—matrixattachmentsparalleltofibronectin-containingextracellularfibers,Thesemoleculestructureswereproposedfortheterm“3D—matrixadhesions.”Thesestructuresincelladhesion,migration,signaling,andcytoskeletalfunctionisdifferentfromstudiesonplanar2Dtissueculturesubstrates.Wepr
3、esentherethehumanforskinfibroblasts(HFF)formed3D-matrixadhesioncomplexesonthethoroughlyacellularhumanamnioticmatrix(TAHAM).Methods:TAHAMwereproducedbysuspendingdigestionwithtrypsin.TheHFFwereseededon6wellplate,matrigelandTAHAMindividually.11helightmicroscope,scanningelectronicmicr
4、oscope,immunohistochemistryandimmunofluorescencewereusedtoobservethemicro-structuresanddetectionthetypel,III,IV,VIcollagen,laminin,fibronectin,TGF-pl’TGF—p2,FGFoftheTAHAM.Deoxyribonucleicacid(DNA)contentoffreshHAMandTAHAMWritsquantifiedusingHoechst33258dye.Phasecontrastmicroscopew
5、asengagedtoobservethemorphologyofHFEThetimelapseCCDandthetraceanalysissoftwarewereemployedtoprescribethecellmigration.The3Dadhesionfociwereindentifiedbythelaserconfocalmicroscope.ThestrainoftheTAHAMWastestedbytheuniversalmechanicaltestinginstrument.Results:ThefibersoftheTAHAMwerei
6、ntact,typeI,III,IV,VIcollagen,laminin,fibronectinwerepositive,TGF—pl,TGF-p2,FGFwerenegative.HFFwerebipolarextended,spindleshaped,formmultilayercellclustersnetworksandinvadededintothematrix.Hoechst33258dyeconfirmedonTAHAMsurfacewithoutcellsandresidualdebris.TAHAMwascomparedwithfres
7、hamnioticmembraneinthetransitionalstress,thetransitionalstrain,failurestress,failurestrain,thedifferenceWasnotstatisticallysignificant(Pvalues>0.05)(transitionalstress:t=-1.83,P=0.08;transitionalstrain:t=-1.07,P=O.30;failurestress:t=1.57,P=0.13;failurestrain:t=一1.81,P=O.08).HFFsee
8、dedontheTAHAMfor24h,thecellsexten
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