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1、DiscStackCentrifugeOperatingParametersandTheirImpactonYeastPhysiology111,2PaulH.Chlup,DominicBernardandGrahamG.StewartABSTRACT•reducingtheyeastquantityfromgreenbeerbeforethestartofsecondaryfermentationJ.Inst.Brew.114(1),45–61,2008•beerrecoveryfromcroppedy
2、eastHydrodynamicstressesimposedonbrewingcellsofSaccharo-•separationofthehotbreakafterwortboilingmycescerevisiaeduringbeerprocessingcanhaveadetrimental•removalofcoldbreakandyeastattheendofimpactonbeerquality.Theuseofcentrifugeshasbecomeanmaturation.efficie
3、ntwaytoincreasebrewerythroughputastheydecreaseYeastmanagementandhandlingsystemsareinfluen-clarificationtimesandimprovefermenterandtankconditioningefficiency.Theeffectofadiscstackcentrifugeonyeastandbeertialindeterminingthephysiologicalstatusofyeast,sub-se
4、quentfermentationperformanceactivity2andclarifi-physicalstabilityhasbeeninvestigated.Inthisstudy,acom-cationduringlagering/maturation32.Ithasbeendocu-mercialaleyeaststrainhasbeensubjectedtodifferentoperatingconditionsduringcentrifugation.Cellviabilityandi
5、ntracellularmentedthatbeerhazecanresultfromtheyeastcellwallpHdecreasedduetoprocessingconditionsencounteredduringreleasingmannanasitisprocessedduringagitationinyeastcroppingwithacentrifuge.Arelationshiphasbeenestab-storageandcentrifugation8,19,32,36.Theser
6、eportssuggestlishedthatyeastcellwallmannan,anunfilterablehazecon-thathydrodynamicstresseshavethepotentialtoinflictstituent,asafunctionofG-forceandcentrifugationcycles,isre-damagetotheyeastcellwallduringbeerproductionandleasedfromthecellwallwhileconcurrent
7、ly,particlesizesbe-consequentlyincreasebeerhaze.However,intermediatetween0.5-2.8μmandbeerhazeincreased.Furthermore,yeasthazeformationduetoagitation,centrifugationandtheirintracellularglycogenandtrehaloselevelsweredepletedasaresultofcentrifugation.relevant
8、operatingparametersstillremainofconsiderableinteresttobrewers.Duringthebrewingprocess,yeastisKeywords:beerhaze,centrifuge,flowcytometry,g-force,subjectedtonumerousfactorsthatindividuallyorcollec-glycogen,intracellul
