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1、AgriculturalScience&Technology,2012,13(5):1021-1027Copyright訫2012,InformationInstituteofHAAS.AllrightsreservedSoilandFertilizerEffectsofArbuscularMycorrhizalFungusandOrganicSubstrateontheMolecularDiversityofMicrobesinSweetPepperRhizosphereunderProtectedCultivation*ChaoxingHE,Lin
2、chuangWANG,ZhibinZHANGInstituteofVegetablesandFlowers,ChineseAcademyofAgriculturalSciences,Beijing100081,Chinasuchasthelowproductivity,con-AbstractDenaturinggradientgelelectrophoresis(DGGE)wasusedtostudytheef-fectsofdifferentsoiladditives,i.e.controlsoil+Glomusmossea-2(G.M),orga
3、nicstraintscausedbythecontinuoussubstrate,organicsubstrate+G.M,onmicrobialmoleculardiversityinsweetpeppercropping,imbalanceinsoilmicrobiota,rhizosphereunderprotectedcultivation.Resultsshowedthatfollowingtheinoculationsoilsecondarysalinization,etc.DuringofG.M,thetotalnumberofbact
4、erialspeciesincreased,withtheappearanceandthepreviousyears,theapplicationofdisappearanceofsomebacterialspecies.Somebacteria,thoughdetectedinalltreatments,showedincreasedcell/clonenumberswiththeadditionofarbuscularmyc-someorganiccultivationtechniquesinorrhizalfungusG.M.Theorganic
5、substratefavoredthegrowthandpropagationofprotectedvegetableproductioninte-somebacteriaandwasadvantageoustotheestablishmentofsomedominantmicro-gratedwiththestrawandmanurere-bialflora.ResultedfromthesimilarityanalysesoftheDGGEprofile,itwasobviousturningintothefield.Withthesetech-t
6、hatorganicsubstratehadmoreinfluenceonthediversityofrhizospheremicrobialflorathantheinoculationofarbuscularmycorrhizalfungusG.M.Meanwhile,bacterialniques,problemsinprotectedcultiva-diversityinthecontrolsoilinoculatedwithG.Mwashigherthantheorganicsoil.Ittion,e.g.soilcrust,frequent
7、epidemicwasthusconcludedthattheinoculationofG.Mchangedthestructureofmicrobialofsoil-bornediseases,continuouspopulationinsweetpepperrhizosphereandincreasedthesimilarityoftherhizo-croppingconstraintsandundergroundspherebacterialspecies.KeywordsArbuscularmycorrhizalfungi;Organicsoi
8、lsubstrate;Denaturinggradientwaterpollution,are
