610-Superdex 200 HR 1030

《610-Superdex 200 HR 1030》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、Superdex200HR10/30INSTRUCTIONSSuperdex™200HR10/30isaprepackedcolumnQualitycontroltestsforhighperformancegelfiltrationofproteins,ToguaranteethequalityofSuperdex200HRDNAfragments(<200bp)andotherbiomolecules.10/30,theefficiencyofeachcolumnistested.EachThecolumncombinesthesuperiorsepar

2、ationmediabatchundergoesafunctiontesttoensurepropertiesofSuperdex200withtheadvantagesofreproducibleresults.anoptimallypackedhighperformancecolumn.ConnectingthecolumntoUnpackingÄKTA™designSystemsPleasecheckthedeliveryagainstthislist.Thecolumnisdeliveredwitharubbertubing,DesignationC

3、odeNoNo.suppliedconnectingtheoutletandinlet.Thecolumnisequilibratedinethanolataconcentrationof20%Superdex200HR10/3017-1088-011Wrench19-7481-011ondelivery.FilterKitHR1018-3575-0111.Beforeconnectingthecolumn,startthepumpFiltertool18-3590-011Instructionsandremoveallairinyoursystem,inp

4、articularintubingsandvalves.Stopthepump.2.MountthecolumnverticallyinacolumnColumndescriptionholder,removetherubbertubingandTheHR10/30columnhasaninternaldiameterofconnectors.10mm.Theheightofthepackedbedis30–31cm.3.Connecttheshorterpreflangedtubing(theThetotalbedvolumeisapproximately

5、24ml.outlet)tothedetectorortothelowercolumnSuperdex200HR10/30ispackedtothehighestselectionvalveV2usingunionFPLCfemale/standardsbyAmershamPharmaciaBiotechandHPLCmale.eachcolumniscarefullytestedregardingnumberoftheoreticalplatespermetre.4.Connectthelongerpreflangedtubing(theinlet)toa

6、valveforsampleinjectionandelutionorSuperdex200isproducedbythecovalentbondingconnectthecolumntotheupperselectionvalveofdextrantohighlycross-linkedporousagaroseV3.beads.TheseparationpropertiesofthecompositemediumispredominantlydeterminedbytheConnectingthecolumntoFPLC™dextrancomponent

7、.ThesteepselectivitycurvegivesexcellentresolutionofproteinsandpeptidesorHPLCsystemsinthemolecularweightrange10000–600000.Superdex200HR10/30canbeusedwithanySuperdex200HR10/30ischemicallystableoverchromatographysystemifthepumpcanprovidethepHrange3–12duringnormaluseandovertheprecisean

8、daccurateflowwithinboththe