Cell-Base ELISA试剂盒

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1、R&Dsystems公司出品的可取代WB的Cell-BaseELISA试剂盒检测蛋白磷酸化，可以不用天天做WesternBlot。可以不用裂解细胞、不用电泳、不用转膜，可以一次检测96个样品，只需要有一台荧光酶标仪，用优宁维公司代理的R&Dsystems公司的Cell-BasedELISA就可以完成。该试剂盒是双荧光检测手段，可在完整细胞条件下替代传统的WB方法同时检测2个生物分子。不需要裂解细胞，更简单，更灵敏。原理如下：试剂盒组分：²与目的蛋白结合的抗体²与Normalization蛋白特异结合的抗体²HRP标记的二抗²AP标记的二抗²与

2、HRP特异结合的F1物质²与AP特异结合的F2物质²洗脱液²封闭液²封板膜²96孔板试剂盒优势：§不用裂解细胞§至少10,000个细胞每孔§可同时检查两种蛋白§Designallowsfornormalizationofwell-to-wellvariations§即用型kit，尽量减少客户优化体系§两种检测方法§Suitableforhigh-throughputanalysisofnon-adherentcells§是替代WB和双抗夹心ELISA试剂盒方法的理想选择§提供6种基础试剂盒，可任意组装自己的试剂盒如下是磷酸化EGFRcell-

3、baseelisakit的结果示例：产品信息有：CATALOG#DESCRIPTIONSIZEKCB1095HumanPhospho-EGFR(Y1068)Cell-BasedELISA1KitKCB887Phospho-Akt(S473)PanSpecificCell-BasedELISA1KitKCB1935Human/MouseTotalHIF-1alphaCell-BasedELISA1KitKCB1018Phospho-ERK1(T202/Y204)/ERK2(T185/Y187)Cell-BasedELISA1KitKCB2288

4、Human/Mouse/RatPhospho-HistoneH2AX(S139)Cell-BasedELISA1KitKCB7226Phospho-RelA/NFkBp65(S536)Cell-BasedELISA1KitKCB2720HumanPhospho-Tie-2(Y992)Cell-BasedELISA1KitKCB3226Phospho-Smad3(S423/S425)/Smad2(S465/S467)Cell-BasedELISA1KitKCB4809Human/Mouse/RatPhospho-IkB-alpha(S32/S3

5、6)Cell-BasedELISA1KitKCB5677HumanPhospho-ErbB3/Her3(Y1262)Cell-BasedELISA1KitKCB4607Human/MousePhospho-STAT3(Y705)Cell-BasedELISA1KitKCB2894HumanPhospho-STAT1(Y701)Cell-BasedELISA1KitKCB7660Phospho-Smad1(S463/S465)/Smad5(S463/S465)Cell-BasedELISA1KitKCB8963Human/MousePhosph

6、o-p70S6Kinase(T389)Cell-BasedELISA1KitKCB2316HumanPhospho-PDGFRbeta(Y1021)Cell-BasedELISA1KitKCB2510Human/Mouse/RatPhospho-CREB(S133)Cell-BasedELISA1KitKCB4418HumanPhospho-ErbB4/Her4(Y1188)Cell-BasedELISA1KitKCB4198Human/MouseTotalCOX-2Cell-BasedELISA1KitKCB001Cell-BasedELI

7、SABaseKit1GoatAnti-MouseIgGAlkPhosandGoatAnti-RabbitIgGHRPAffinityPurifiedPAbs1KitKCB5126Human/Mouse/RatPhospho-FRS2(Y436)Cell-BasedELISA1KitKCB006Cell-BasedELISABaseKit6GoatAnti-MouseIgGHRPandGoatAnti-RabbitIgGAlkPhosAffinityPurifiedPabs1KitKCB8871Phospho-Akt(T308)PanSpeci

8、ficCell-BasedELISA1KitKCB3717Human/MousePhospho-STAT6(Y641)Cell-BasedELISA1KitKCB4