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1、AgriculturalScience&Technology,2011,12(4):527-529Copyright2011,InformationInstituteofHAAS.Allrightsreserved.AgronomyStudiesontheMicrobialCommunityintheRhizosphereSoilofDongxiangWildRice*DAIFe,iHUANGYunhong,PENGWeimeng,LILuming,LONGZhongerCollegeo
2、fLifeSciences,JiangxiNormalUniversity,Nanchang330022Abstract[Objective]TheamiwastostudythecharacteristicsofmicrobialcommunityintherhizospheresoilofDongxiangwildrice(OryzarufipogonGrif.f).[Method]Themicrobialbiomasscarbonandnitrogenwasestmiatedbythec
3、hloroformfumigationextractionmethod;themicrobialcommunitycompositionandNitrogencyclingmicrobialfunctionalgroupswereinvestigatedbytheDilutionplateculturemethodandthemostprobablenumbermethodsrespectively.[Result]Themicrobialbiomasscarbonandnitrogen,inr
4、hizospheresoilofDongxiangWildRicewas83.0218.23mg/kgsoiland16.982.54mg/kgsoi,lwhichwaslowerthanthatofordinarycultivatedrice;Therelationshipbetweenthenumberofculturablemicrobialgroupswasbacteria>actinomycetes>fung,iandtheNitrogencyclingmicrobialphysi
5、ologicalgroupswasasthefolowing:ammonifyingbacteria>aerobicazotobacteria>denitrobacteria>anaerobicazotobacteria>nitrobacteria>nitrosobacteria.[Conclusion]ThemicrobialcommunityintherhizospheresoilofDongxiangWildRicewasdifferentfromthatoftheordinarycult
6、ivatedrice.KeywordsDongxiangWildRice;Rhizospheremicroorganisms;CommunitycompositionOryzarufipogonGrif.fisakindofpreciousseedsreinto1mlchloroform(inthemeantmie,takingnonchloroformsourcesinthewholeworld.Itisasignificantmaterialbasisforfumigationasc
7、omparison),fumigating24hinthedarkroom[1-2]thedevelopmentofricebreeding.DongxiangOryzarufiandthenmimerginginto40ml(14ratioofsoilandwater)pogonGrif.fisthealreadyfoundmostnorthcommonwildriceKSO,andmimediatelytousecontentanalysismethodand24inChinaandeve
8、nintheworldsofar.Itcarriesrichresistantcolormietrymethodtomeasurethecarbonandnitrogeninmi[13]characteristicssuchasantidisease,antidrough,tandanticrobiomassintheextractedliquid.coldnessetc.IthasbeenlistedassecondgradenationalproNumberofrh