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1、动物学杂志ChineseJournalofZoology2008,43(3):102～107池蝶蚌胚胎发育与繁殖季节性腺的观察①①3②①②①余颖洪一江邱齐骏王军花徐毛喜李云娟(①南昌大学生命科学学院南昌330031;②洪门水库开发公司江西抚州334000)摘要:以池蝶蚌(Hyriopsisschlegeli)育儿囊中单细胞胚胎为材料,通过连续观察、人工体外培养等方法,对繁殖季节池蝶蚌的生殖腺特性、生殖细胞形态、胚胎发育过程等进行了观察。结果表明,年满4冬龄池345蝶蚌卵巢的相对怀卵量为(2125±1109)×
2、10粒Pg,绝对怀卵量为1193×10～1103×10粒;性腺指数为24109%±0133%;在体外用自制培养液培养的胚胎,部分能正常分裂发育至桑椹期,其胚胎发育过程经历了4个时期,即卵裂期、囊胚期、原肠期、钩介幼虫期。在18～23℃的水温下,胚胎发育历时约12d;胚胎发育的最适水温为20～30℃。此外,通过池蝶蚌外鳃的特征可初步判断胚胎发育的时期。研究结果可为池蝶蚌人工繁殖、新品种培育及种质资源保护等提供理论依据。关键词:池蝶蚌;胚胎发育;怀卵量;生殖细胞中图分类号:Q13214文献标识码:A文章编号:02
3、5023263(2008)032102206EmbryonicDevelopmentandBreading2seasonGonadinHyriopsisschlegeli①①3②①②①YUYingHONGYi2JiangQIUQi2JunWANGJun2HuaXUMao2XiLIYun2Juan(①CollegeofLifeScience,NanchangUniversity,Nanchang330031;②HongmenReservoirExploitationCompany,Fuzhou334000,Ch
4、ina)Abstract:Thecharacteristicsofgonadalstructure,gamete(spermandegg)morphologyandembryonicdevelopmentofHyriopsisschlegeliwerestudiedbycontinuousobservationandinvitroculture.Theresultsshowedthattheabsolute453fecunditywas1193×10-1103×10eggsandtherelativefecu
5、nditywas(2125±1109)×10eggsPg.Thegonadindex(GI)was24109%±0133%.Theembryoculturedinvitrocoulddevelopsuccessfullytoblastocystinnutrientsolutionpreparedbyourselves.Embryonicdevelopmentandprocessweredescribed.TheembryodevelopmentofH.schlegeliwasdividedintofourst
6、ages:cleavage,blastocyst,gastrulaeandglochidia.Theembryonicdevelopmenttook12dayswhenthewatertemperaturerangedbetween18-23℃.Theoptimumtemperaturerangefortheembryonicdevelopmentwas20-30℃.Asobserved,theglochidiaofthisspecieswerehookedandtherewererelationsbetwe
7、enembryonicdevelopmentstagesandtheoutergillcharactersofthefemale.Ourresultswillprovidevaluabledataforartificialreproduction,germplasmresourceconservationandbreedinginfreshwaterpearlmussel.Keywords:Hyriopsisschlegeli;Embryonicdevelopment;Fecundity;Germcell国内
8、外关于贝类胚胎发育已有很多报道。卵裂期、囊胚期、原肠期、担轮幼虫期及D形幼[1]Madrones2Ladja分别采用两种不同的方法对比观察了海月科窗贝(Placunaplacenta)产卵、胚基金项目农业部948项目(No.20042T9);[2]3通讯作者,E2mail:yjhong2008@163.com;胎及幼体发育等过程的异同。李琼珍等对海第一作者介绍余颖,女,硕士;研究方向:水生动物遗传育
