动物细胞蛋白质的提取和含量的测定

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1、0.50.40.30.20.10.00.00.10.20.30.40.5图1Lowry法测定蛋白质浓度标准曲线0.40.30.20.10.00.000.050.100.150.200.250.30图2Lowry法测定蛋白质浓度标准曲线0.50.40.30.20.10.00.00.10.20.30.40.5图3Lowry法测定蛋白质浓度标准曲线Equationy=a+b*xAdj.R-Square0.99523ValueStandardErrorBIntercept0.011970.01306BSlope1.009120

2、.04032Equationy=a+b*xAdj.R-Square0.91653ValueStandardErrorBIntercept0.043140.04088BSlope1.009430.13501Equationy=a+b*xAdj.0.98897R-SquareValueStandardErrorBIntercept-0.00270.01621BSlope1.4230.086620.50.40.3B0.20.10.00.00.10.20.30.40.5图4考马斯亮蓝法测定蛋白质浓度标准曲线0.50.40.30

3、.20.10.00.00.10.20.30.4图5考马斯亮蓝法测定蛋白质浓度标准曲线Equationy=a+b*xAdj.R-Square0.99474ValueStandardErrorBIntercept0.01260.0099BSlope1.1120.0404Equationy=a+b*xAdj.R-Square0.97709ValueStandardErrorBIntercept0.027190.02074BSlope1.002570.068490.400.350.300.250.200.150.100.050

4、.000.00.10.20.30.40.50.6图6紫外吸收法测定蛋白质浓度标准曲线Equationy=a+b*xAdj.R-Square0.98185ValueStandardErrorBIntercept0.004140.01353BSlope0.575570.034930.400.350.300.250.200.150.100.050.000.00.10.20.30.40.50.6图6紫外吸收法测定蛋白质浓度标准曲线Equationy=a+b*xAdj.R-Square0.99513ValueStandardEr

5、rorBIntercept0.001810.00761BSlope0.566230.01979