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1、BMCGenomicsBioMedCentralMethodologyarticleOpenAccessComparisonofnextgenerationsequencingtechnologiesfortranscriptomecharacterizationPKerrWall1,JimLeebens-Mack2,AndréSChanderbali3,AbdelaliBarakat4,ErikWolcott1,HaiyingLiang4,LenaLandherr1,LynnPTomsho5,YiHu1,JohnECarlson4,HongMa1,StephanCSchuster5,D
2、ouglasESoltis3,PamelaSSoltis6,NaomiAltman7andClaudeWdePamphilis*1Address:1DepartmentofBiology,InstituteofMolecularEvolutionaryGenetics,andTheHuckInstitutesoftheLifeSciences,ThePennsylvaniaStateUniversity,UniversityPark,PA16802,USA,2DepartmentofPlantBiology,UniversityofGeorgia,Athens,GA30602,USA,3
3、DepartmentofBiology,UniversityofFlorida,POBox118526,Gainesville,FL,32611,USA,4TheSchoolofForestResources,DepartmentofHorticulture,andHuckInstitutesoftheLifeSciences,PennsylvaniaStateUniversity,323ForestResourcesBuilding,UniversityPark,PA16802,USA,5CenterforComparativeGenomics,CenterforInfectiousD
4、iseaseDynamics,ThePennsylvaniaStateUniversity,UniversityPark,PA16802,USA,6FloridaMuseumofNaturalHistory,UniversityofFlorida,P.O.Box117800,Gainesville,FL,32611,USAand7DepartmentofStatisticsandTheHuckInstitutesoftheLifeSciences,ThePennsylvaniaStateUniversity,UniversityPark,PA16802,USAEmail:PKerrWal
5、l-pkerrwall@psu.edu;JimLeebens-Mack-jleebensmack@plantbio.uga.edu;AndréSChanderbali-achander@botany.ufl.edu;AbdelaliBarakat-aub14@psu.edu;ErikWolcott-eww5024@psu.edu;HaiyingLiang-hliang@clemson.edu;LenaLandherr-lll109@psu.edu;LynnPTomsho-lap153@psu.edu;YiHu-yxh13@psu.edu;JohnECarlson-jec16@psu.ed
6、u;HongMa-hxm16@psu.edu;StephanCSchuster-scs@bx.psu.edu;DouglasESoltis-dsoltis@botany.ufl.edu;PamelaSSoltis-psoltis@flmnh.ufl.edu;NaomiAltman-naomi@stat.psu.edu;ClaudeWdePamphilis*-cwd3@psu.edu*CorrespondingauthorPublished:1August2009Received:1August2008Accepted:1August2009BMCGenomics2009,10:347do
7、i:10.1186/1471-2164-10-347Thisarticleisavailablefrom:http://www.biomedcentral.com/1471-2164/10/347©2009Walletal;licenseeBioMedCentralLtd.ThisisanOpenAccessarticledistributedunderthetermsoftheCreativeCommonsAttributionL