Southern, Northern and Western blotting 课件

《Southern, Northern and Western blotting 课件》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、生物秀实验频道——生命科学实验中心www.bbioo.com/bio101SouSoutthheerrnn,,NNoortrthheerrnnaandndWesWestteerrnnbblloottttiingng生理所黃阿敏生物秀—专心做生物！www.bbioo.com易生物-领先的生物医药商务平台www.ebioe.com生物秀论坛-学术交流,资源共享,互助社区www.bbioo.com/bbs/生物秀论坛——学术交流、资源共享、互助社区www.bbioo.com/bbs生物秀实验频道——生命科学实验中心www.bbioo.com/bio101Compari

2、sonofSouthern,Northern,andWesternanalysesofGeneX生物秀论坛——学术交流、资源共享、互助社区www.bbioo.com/bbs生物秀实验频道——生命科学实验中心www.bbioo.com/bio101SouthernhybridizationnFirstdescribedbyE.M.Southernin1975.nApplicationsofSouthernhybridizationnRFLP’s,VNTR’sandDNAfingerprintingnCheckingofthegeneknockoutmicenThe

3、flowchartofSouthernhybridization生物秀论坛——学术交流、资源共享、互助社区www.bbioo.com/bbs生物秀实验频道——生命科学实验中心www.bbioo.com/bio101SouthernhybridizationTransferbuffer生物秀论坛——学术交流、资源共享、互助社区www.bbioo.com/bbs生物秀实验频道——生命科学实验中心www.bbioo.com/bio101DetectionofanRFLPbySouthernblotting生物秀论坛——学术交流、资源共享、互助社区www.bbioo.c

4、om/bbs生物秀实验频道——生命科学实验中心www.bbioo.com/bio101Detectionofthesickle-cellglobingenebySouthernblotting生物秀论坛——学术交流、资源共享、互助社区www.bbioo.com/bbs生物秀实验频道——生命科学实验中心www.bbioo.com/bio101Morethan3000humangeneticdiseasesareattributabletosingle-genedefects.Inmostofthesethemutationisrecessive:thatis,it

5、showsitseffectonlywhenanindividualinheritstwodefectivecopiesofthegene,onefromeachparent.Onegoalofmodernmedicineistoidentifythosefetusesthatcarrytwocopiesofthedefectivegenelongbeforebirthsothatthemother,ifshewishes,canhavethepregnancyterminated.Insickle-cellanemia,forexample,theexactn

6、ucleotidechangeinthemutantgeneisknown(thesequenceGAGischangedtoGTGataspecificpointintheDNAstrandthatcodesfortheβchainofhemoglobin).Forprenataldiagnosis,twoDNAoligonucleotidesaresynthesized-onecorrespondingtothenormalgenesequenceintheregionofthemutationandtheothercorrespondingtothemut

7、antsequence.Iftheoligonucleotidesarekeptshort(about20nucleotides),theycanbehybridizedwithDNAatatemperatureselectedsothatonlytheperfectlymatchedhelixwillbestable.SucholigonucleotidescanthusbeusedaslabeledprobestodistinguishbetweenthetwoformsofthegenebySouthernblottingonDNAisolatedfrom

8、fetalcellsco