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1、JournalofGeneralMicrobiology(1980),116,11I-131.PrintedinGreatBritain111TheinvivoThree-dimensionalFormofaPlantMycoplasma-likeOrganismbytheAnalysisofSerialUltrathinSectionsByHENRYWATERSANDPETERHUNT2*CoconutIndustryBoard,P.O.Box204,Kingston10,JamaicaBotanyDepartment,Universityofthe
2、WestIndies,Kingston7,Jamaica(Received24April1979)Mycoplasma-likeorganisms(MLO)associatedwithlethalyellowingdiseaseofcoconutpalmsexhibitedarangeofdifferentmorphologiesevenwithinthesamesieveelement.Themorphologieswererevealedbygraphicreconstructionfromribbonsofserialultrathinsecti
3、onscontaining45sections.Fivedifferentmorphotypeswererecognizedamongstthe120organismsreconstructed.MLOcouldbesaccate,erythrocyte-like,cylindrical,monili-formorfiliform.Thedifferentmorphotypesshoweddifferencesinlineardimensionsapartfromtheirparticularmorphologicalcharacteristics.S
4、accateMLOweregenerallythesmallest,themaximumlengthbeing1pm,whilstthefiliformorganismscouldbemorethan16,umlong.ThemorphologiesoftheMLOwereanalogoustothosenowrecognizedasusualforMycoplasmaandAcholeplasmaspeciesinvitroduringthelaterstagesofdevelopment.Comparisonoftheindividualprofi
5、leshapesusedinthereconstructionswiththoseofMLOfromotherdiseasedplantssuggestthatmanypreviousinterpretationsofMLOmorphologymayhavebeentoosimple.Withtheinsightprovidedbytheunder-standingoftherelationshipbetweenprofileshapeandthree-dimensionalform,theultra-structureofindividualprof
6、ilesisshowntobeanimportantindicatorofform.INTRODUCTIONObservationsofintactmycoplasmastakenfromcultureshowthatalthoughtheyoccurmostfrequentlyassimplespheresorfilamentousbodiestheyarecapableofbecomingfreelybranchedorevengivingrisetomycelialcomplexes(Razin,1978;Tully,1978).Othermod
7、ificationsofform,suchasringsandbeadedchains,havealsobeendescribed.Theelucidationofmorphologyinvitrobegannearly70yearsagobuttheexceptionallydetailedlightmicroscopicstudiesofTurner(1935)providesomeofthebestearlyillustrations.Morerecently,theuseofsimilarormoresophisticatedtechnique
8、shasgenerallyconfirmedtheseearlystudiesdespites
