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1、作物学报ACTAAGRONOMICASINICA2010,36(3):376384http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2010.00376粳稻叶绿素含量QTL与其合成降解相关基因的比较分析121,*1姜树坤张喜娟徐正进陈温福12沈阳农业大学/农业部作物生理生态遗传育种重点开放实验室,辽宁沈阳110161;辽宁省丹东农业科学院,
2、辽宁凤城118109摘要:为剖析水稻叶绿素不同时期的表达规律及后期持绿的遗传机制,以沈农265和丽江新团黑谷的粳-粳交重组自交系为材料,对水稻分蘖期、抽穗期和成熟期的叶绿素含量以及生育后期的持绿能力进行QTL定位分析。检测到5个控制分蘖期叶绿素含量的QTL、7个控制水稻抽穗期叶绿素含量的QTL和10个控制成熟期叶绿素含量的QTL,分布在除第5染色体以外的11条染色体上。比较它们与编码叶绿素合成及降解过程中的重要酶的基因发现,虽然生育前期检测到的QTL较少,但对应的叶绿素合成降解相关基因却较多。随生育期的推移
3、,检测到的QTL数目增多,但对应的叶绿素合成降解相关基因却减少。暗示生育前期大多数叶绿素合成(降解)相关基因表达的水平差异不大,后期控制叶绿素合成降解的个别关键基因表达水平增加。并以此为基础提出了叶片生育后期持绿的两种可能遗传基础。关键词:粳稻;叶绿素含量;持绿性;数量性状位点;叶绿素合成降解ComparisonbetweenQTLsforChlorophyllContentandGenesControl-lingChlorophyllBiosynthesisandDegradationinJaponica
4、Rice(OryzasativaL.)121,*1JIANGShu-Kun,ZHANGXi-Juan,XUZheng-Jin,andCHENWen-Fu1KeyLaboratoryofCropPhysiology,Ecology,GeneticsandBreeding,MinistryofAgriculture/ShenyangAgriculturalUniversity,Shenyang110161,2China;DandongAcademyofAgriculturalSciences,Fengcheng
5、118109,ChinaAbstract:Tounderstandtheexpressionpatternsofchlorophyllcontentrelatedgenesatdifferentstagesandgeneticmechanismsofstay-greenatlaterstage,weanalyzedtheQTLscontrollingchlorophyllcontentatthestagesoftillering,headingandmaturitybyemploying126recombi
6、nantinbredlines(RILs)derivedfromacrossbetweentwojaponicaricecultivars,Shennong265andLijiangxintuanheigu.Five,seven,andtenQTLscontrollingchlorophyllcontentsattilleringstage,headingstageandmaturitystageweredetected,respectively.Theyweredistributedonallricech
7、romosomesexceptchromosome5.ComparisonoftheQTLsandthegenesunderlyingthekeyenzymesofchlorophyllbiosynthesisanddegradationrevealedthatrelativelymoreQTLsde-tectedatearlierstageco-locatedwiththegenesrelatedtochlorophyllbiosynthesisanddegradation.Withthegrowthst
8、agegoingon,moreQTLsweredetectedbutonlyafewoftheminvolvedinchlorophyllbiosynthesisanddegradation.Theresultssuggestedthattheexpressionlevelofmostgenesrelatedtochlorophyllbiosynthesis(degradation)hadnodifference
