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1、新生兔重度胎粪吸入模型的制备作者：周海燕,武荣单位：滁州安徽医科大学滁州临床学院儿科教研室【摘要】目的观察不同浓度胎粪悬混液对新生兔的影响，建立合适的机械通气胎粪吸入模型。方法21只新生兔，随机分成25mg/ml浓度组,45mg/ml浓度组,65mg/ml浓度组以及对照组，分别由气管导管注入胎粪悬混液4ml/kg（对照组注入等量生理盐水），达到通气指征后，予以机械通气。观察各组8h内病死率、胎粪灌入前及胎粪灌入后30min肺顺应性（Cydn）、血气、氧合指数、肺组织大体病理情况及肺损伤病理评分。结果25mg/ml组肺损伤小，8h内均未达到机械通气指征；45mg/ml组均在0.5h到机
2、械通气指征，肺损伤较小；65mg/ml组在0.5h均达到机械通气标准，其病理显示肺损伤严重，实变及出血灶较大，病死率高。结论45mg/ml组达到机械通气指征的时间较理想，肺损伤程度适中，适合做为机械通气模式等相关研究的重度胎粪吸入模型。【关键词】胎粪吸入；机械通气；模型；新生兔Modelofseveremeconiumaspirationinnewly-bornrabbitsZhouHaiyan,WuRongDepartmentofPediatrics,ChuzhouClinicalCollegeofAnhuiMedicalUniversity,Chuzhou239000,China
3、［Abstract］ObjectiveInvestigatetheeffectsofmeconiumsolutionsonnewly-bornrabbits,andtoestablishasuitablemodelofmeconiumaspirationwhichneedstoapplymechanicalventilation.Methods21newly-bornrabbitswererandomlydividedinto25mg/mlconcentrationgroup(n=6),45mg/mlconcentrationgroup(n=6),65mg/mlconcentrati
4、ongroup(n=6)andthecontrolgroup(n=3).Meconiumsolutionswereinjectedintotheendotrachealtubeby4ml/kginthefirstthreegroupswhilecontrolgroupinjectedequivalentsaline.Mechanicalventilationwhenachievedtheventilationindications.Observedthemortalitywithin8hoursineachgroup,lungdynamiccompliance(Cydn)inthep
5、re-and30minutesaftermeconiumpoured,bloodgases,oxygenationindex,lungtissueingeneralpathologyandlunginjuryscore.Results25mg/mlconcentrationgroupwereminimallunginjury,andfailedtomeetwithintheindicationsformechanicalventilationin8h;45mg/mlconcentrationgroupwereabletoachievetheindicationsin0.5h,andh
6、adlesslunginjury;65mg/mlconcentrationgroupallreachedtheindicationsin0.5h,itspathologyrevealedseverelunginjury,pulmonaryconsolidationandhemorrhagefocuswerelarger,andthemortalityratewerehigher.Conclusion45mg/mlconcentrationgroupachievedtheindicationsofmechanicalventilationinidealtime,thedegreeoft
7、helunginjuryisappropriate.Thisgroupisasuitableoneasaseveremeconiumaspirationmodelwhichcanbeappliedintheresearchrelatedtomechanicalventilation.［Keywords］Meconiumaspiration；Mechanicalventilation；Model;Newbornrabbit胎粪吸入综合征（MAS）是新生儿常见
