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1、妊娠中期弓形虫感染对胎鼠生长发育影响【摘要】目的探讨弓形虫感染妊娠中期BALB/c小鼠对胎鼠生长发育以及胎盘系数的影响。方法在妊娠第8d采用腹腔注射法建立弓形虫感染的动物模型,于妊娠第12,14,16,18d分批处死孕鼠,分离胎盘胎鼠,解剖显微镜下观察胎鼠存活情况,称量胎盘和胎鼠重量,测量胎鼠体长和尾长。结果随着妊娠天数的增加,实验组孕鼠吸收胎明显增多,出现死胎和流产等,正常对照组未见异常;正常对照组胎鼠重量、体长和尾长增加明显,胎盘重量呈现先增后减的趋势,胎盘系数呈降低趋势,实验组变化趋势不及
2、正常对照组明显,组内比较差异均有统计学意义(P<0.05)。相同时间点的比较显示,除12d的胎盘胎鼠重量14,16d的胎盘系数和14d的胎鼠体长外,差异有统计学意义(P<0.05)。结论弓形虫感染妊娠中期BALB/c小鼠可导致胎盘功能障碍,胎鼠生长发育受限,且随着感染天数的增加,可出现死胎和流产等病理性妊娠的发生。【关键词】刚地弓形虫;胎盘系数;体长;尾长EffectongrowthanddevelopmentoffetusinfectedwithToxoplasmagondiiduringse
3、condtrimesterpregnancyinratsRAOHuaxiang,HOUYuying,LIUZhishen,etal.InstituteofMedicalParasitology,ShanxiMedicalUniversity(Taiyuan030001,China)Abstract:ObjectiveToinvestigatethegrowthanddevelopmentoffetusandplacentalcoefficientofBALB/cmiceinfectedwi
4、thToxoplasmagondiiduringthesecondtrimesterpregnancy.MethodsToestablishtheanimalmodelsbyintraperitonealinjection(i.p.)methodatthe8thdayofpregnancy,thenseparatetheplacentasandfetusatthe12th,14th,16th,18thday,respectively.Thesurvivalrateoffetuswasobserv
5、edbyanatomicalmicroscope.Theweightofplacentasandfetuswereweightedbyelectronicbalance,andthebodylengthandtaillengthweremeasuredbyslidingcaliper.ResultsItwasshowedthatthenumberofabsorptionfetusincreasedapparentlyalongwiththatofthepregnantdaysintheexper
6、imentalgroup,ofwhichthedeathoffetusandabortionweremorethanthatofthenormalcontrolgroup.Inthenormalcontrolgroup,theweight,bodylengthandtaillengthoffetuswereincreasedobviousandtheplacentalweightwasincreasedfirstthendecreased,whichcausedthedecreaseofplac
7、entalcoefficient.However,thechangeoffetusandplacentaoftheexperimentalgroupwerenotobviouslycomparedwiththenormalcontrolgroup.Atdifferenttimepoints,therewassignificantdifferenceineachgroup(P<0.05).Atthesametimepoints,theresultsoftheexperimentalgroupwer
8、esignificantdifferentcomparedwiththatofthenormalcontrolgroupexcepttheweightoftheplacentaatthe12thday,placentalcoefficientatthe14thday,andbodylengthatthe14thday(P<0.05).ConclusionTheinfectionofToxoplasmagondiiduringthesecondtrimesterpregnancycancaused