翔科生物蛋白芯片技术在医学研究的应用重庆肿瘤

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1、ScottLuoPh.D.R&DassociateDirectorsluo@raybiotech.comorsluo815@gmail.comCell:13119583381(China)217-721-9160(US)广州瑞博奥生物科技有限公司广州华南生物芯片研究中心Tel:020-32077025Fax:020-32077025Website:www.raybiotech.com.cnOrwww.raybiotech.com蛋白质与抗体芯片在生物医学研究和转化医学中的应用ArraysofDiscovery:TheapplicationofPro

2、teinAntibodyArraysinBiomedicalResearchandTranslationalMedicineRayBio®CSeriesArray:MouseCytokineAntibodyArrayIIIIL-6IL-6KnockoutmouseIL-6WildtypemouseHigh-content(高密度)每张芯片同一样品可同时检测多达数百种指标。OmicsTool(蛋白质组学的工具)可用于分析反应的模式。HighThroughput(高通量)每天完成多至上千样品的测试。Miniaturization微型化节省样品和试剂,实验空

3、间,仪器设备Highsensitivity,specificityandreproducibility高灵敏，高特异性，重复性蛋白芯片是通过芯片矩阵平台同时检测多种蛋白质表达及功能的技术Benefitsofproteinandantibodyarrays蛋白和抗体芯片的优点抗体芯片的载体膜芯片玻璃芯片SandwichMethodDirectLabelMethodCompetitiveMethodLabel-freeMethodA.B.C.D.Signaldetectioninmostantibodyarraysreliesoncommonlabels(b

4、iotin,fluors,orenzymes)attachedtotheantibodyorantigen.Thesandwichmethod(A)requiresanimmobilizedcaptureantibodyandalabeled,in-solutiondetectionantibody.Thismethodhastheadvantagesofexcellentspecificityandsensitivity.Thecompetitivemethod(B)reliesoncompetitivebindingbetweentheanalyte

5、andalabeledantigen.Inthedirect-labelmethod(C),thelabelisattachedtotheanalyteitself,whilethelabel-freemethod(D)reliesonaninherentpropertyoftheanalyte(suchasmassperunitareaorsurfaceplasmonresonance)toproduceasignal.ProcessingQuantibody®DataIL-8NegativeIL-10IL-2IL-4IL-5IL-6IL-13GM-C

6、SFIFNTNFStandardCurvePositiveDataExtraction(ArrayVision,GenePix)StandardCurve(RaybioQ-Analyzer)QuantitativeDataExport全自动化蛋白质芯片检测仪：整合自动加样，清除，孵化，检测，结果分析为一体的仪器，已完成整体设计和样机制作。Automaticproteinchipanalyzer:integrationofsamplehandling,wash,incubation,detectionanddataanalysisintoasingle

7、device.全自动化蛋白质芯片检测仪(Automaticproteinchipanalyzer)Alzheimer’sDiseasethesearchforbiomarkersAlzheimer’sisaprogressive,fatalneurodegenerativebraindisorder–themostcommonformofdementiaDiagnosisismadebyaprocessofeliminationusingacombinationofimaging,cognitiveandneuropsychologicalexamsN

8、ochemistry-baseddiagnosticcurrentlyexist