雌激素在卵巢切除小鼠疼痛调制中的作用

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4、酸性蛋白钙结合蛋白雌激素雌激素受体3．氨丙基．3乙氧基甲硅烷第三军医大学硕士学位论文RoleofestrogeninthepainmodulationofovariectomizedmiceAbstractGenderdifferencesinpainperceptionhavebeendemonstratedinclinicalstudiesandanimalexperiments．EstrogenactionsnowarerecognizedtoOccurviatwodistinctestrogenreceptors(ER)，ERaandER

5、l3arewidelydistributedintheCNSregionrelatedtopainmodulation．TheseanatomicalstructureindicateestrogenactsonERtomodulatepainperception,whereastheunderlyingmechanismisstillinquestion．Herewemainlyexplorethepossibleeffectandpathwayofestrogenmodulatingpainthroughintermittentneurona

6、ctivationinthedorsalspinalcord，painafferentfibersandastrocytesactivation．TheeffectsofestrogenonpainthresholdWasevaluatedwithimmunohistochemitryandpainbehaviourtestandpainscoreinovariectomizedC57B／L6micefollowingestrogenintraperitonealinjections．Thepainmicemodelwasestablishedb

7、yinjectingformalinintothefighthindpaw，C57B／L6micewererandomizedintofourgroups：controlgroup(intactmice)，Shamgroup，Vehiclegroup(ovariectomizedmicewithsesameoilipinjections)andEstrogengroup(ovariectomizedmicewith1713一estradiolipinjections)．Theeffectsofneuronalactivationandpainaf

8、ferentfibersinthedorsalhornofspinalcordwereinvestigatedbymeasurement