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1、中国水稻科学(ChinJRiceSci),2013,27(3):223-230http://www.ricesci.cnDOI:10.3969/j.issn.1001-7216.2013.03.001223抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲李磊1,#薛芗1,#左示敏1陈宗祥1张亚芳1李前前1朱俊凯2马玉银3潘学彪1,*潘存红2,*(1扬州大学农学院,江苏扬州225009;2江苏里下河地区农业科学研究所,江苏扬州225007;3扬州职业大学生化工程系,江苏扬州225009;#共同第一作者;*通讯联系人,E-mail
2、:shuidao@yzu.edu.cn;pancunhong@163.com)SuppressedExpressionofOsAGO1aLeadstoAdaxialLeafRollinginRiceLILei1,#,XUEXiang1,#,ZUOShi-min1,CHENZong-xiang1,ZHANGYa-fang1,LIQian-qian1,ZHUJun-kai2,MAYu-yin3,PANXue-biao1,*,PANCun-hong2,*(1AgriculturalCollege,YangzhouUniversity,
3、Yangzhou225009,China;2LixiaheAgriculturalResearchInstituteofJiangsuProvince,Yangzhou225007,China;3DepartmentofBiochemicalEngineering,YangzhouPolytechnicCollege,Yangzhou225009,China;#Theseauthorscontributeequallytothispaper;*Correspondingauthors,E-mail:shuidao@yzu.edu
4、.cn;pancunhong@163.com)LILei,XUEXiang,ZUOShimin,etal.SuppressedexpressionofOsAGO1aleadstoadaxialleafrollinginrice.ChinJRiceSci,2013,27(3):223-230.Abstract:Argonaute(AGO)proteinisthecoreeffectorsinRNA-inducedsilencingcomplexes(RISCs),playinganndththimportantroleinplan
5、tdevelopment.The2andthe11-12exonfragmentsofOsAGO1awereamplifiedindependentlytomaketwoRNAiconstructs:OsAGO1aI-1EandOsAGO1aI-2E.ToclarifythefunctionofOsAGO1ainricedevelopment,theexpressionofOsAGO1ahasbeensuppressedbyintroducingRNAiconstructstoNipponbarethroughAgrobacte
6、rium-mediatedtransformation.TheresultshowedthatthereducedexpressionofOsAGO1aleadtoadaxialleafrollingwithoutaffectingmainagronomictraits.Keywords:rice;OsAGO1a;polaritydevelopment;rolledleaf;agronomictraits李磊,薛芗,左示敏,等.抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲.中国水稻科学,2013,27(3):223-230
7、.摘要:AGO蛋白是RNA诱导沉默复合体的核心元件,在植物生长发育过程中发挥着重要作用。通过分别特异性地扩增OsAGO1a基因中第2外显子和第11~12外显子的片段,构建了两个干涉载体OsAGO1aI-1E和OsAGO1aI-2E。利用农杆菌介导法转化水稻品种日本晴,抑制OsAGO1a基因的表达,借此研究OsAGO1a的功能及其对水稻生长发育的影响。结果表明,抑制OsAGO1a的表达导致叶片的近轴面卷曲而对其他主要农艺性状的影响不大。关键词:水稻;OsAGO1a;极性发育;卷叶;农艺性状中图分类号:Q786;S511.032文献
8、标识码:A文章编号:1001-7216(2013)03-0223-08AGO(Argonaute)蛋白是RISC(RNA-inducedsiRNA,tasiRNA);AGO4结合重复相关siRNAsilencingcomplex,RNA诱导沉默复合体)的标志(r
