表皮蛋白质及板层小体对激素依赖性皮炎皮肤屏障变化的影响

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1、生华皮肤科杂志2012年2月第45卷第2期ChinJDermatol，February2012，Vo1．45．N0．287·论著·表皮蛋白质及板层小体对激素依赖性皮炎皮肤屏障变化的影响起珏顾华汤访涂颖庞勤张丽何黎【摘要】目的探讨表皮蛋白质、板层小体的变化对激素依赖性皮炎皮肤屏障变化的影响。方法无创性皮肤测试比较60例激素依赖性皮炎患者与4O例正常人，皮肤经表皮水分流失(TEwL)的差异，13例患者及10例正常人配合皮损取材，行HE染色观察皮损组织病理学变化。免疫组化观察患者表皮K6、KIO、K14、K15、兜甲蛋白、丝聚合蛋白、内披蛋白的变化。电镜观察板层小体密度的变化。结果

2、与正常人比较，患者TEWL增加，差异有统计学意义(P

3、dermalproteinsandlameUarbodiesonepidermalbarrieringlucocorficoid-dependentdermatitisQIJue，GUHua，TANGYang,TUYing,PANGQin，ZHANGH，HELi．DepartmentofDermatology,FirstAffiliatedHospitalofKunmingMedicalCoHege，YunnanProvincialInstituteofDermatology,Kunming65oo32．ChinaCorrespondingaumor：HELi。Email：h

4、e1i2662@yahoo．com．cn【Abstract]ObjectiveToexploretheeffectsofepidermalproteinsandlamellarbodiesonskinbarrieringlueocorticoid．dependentdermatitis．MethodsTotMly，60patientswithglucoeorticoid-dependentdermatitisand40normalhumancontrolswereeligibleforthisstudy．AnoninvasivemethodusingTewameterTMwa

5、sappliedtodeterminetransepidermalwaterloss(TEWL)valueintheseSUbjects．Tissuespecimenswereobtainedfromthelesionsof13patientswithglucoeorticoid—dependentdermatitisandnormalskinof10humancontrols．Subsequently，haematoxylinandeosin(HE)stainingwasperformedtoobservethehistopathologicalchanges，immuno

6、histochemistrytodetecttheproteinexpressionsofK6，K10，K14，K15，loricrin，filaggrin，involucrininepidermis．andelectronmicroscopy(EM)toestimatethedensityoflamellarbodiesintissuespecimens．ResultsComparedwiththenormalcontrols，thepatientsdisplayedanelevatedTEWLvalue(P<0．05)。whichsuggestedanimpairedep

7、idermalbarrier．Histopathologyoflesionsrevealednonspecifieinflammatorychangeswithmarkeddifferencesbetweendifferentclinicaltypesofglueocorticoid—dependentdermatitis．ImmunohistochemistryrevealedanattenuatedexpressionofK10，K14，lorierin，filaggrin，involucrinan