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1、生态环境学报2009,18(2):447-452http://www.jeesci.comEcologyandEnvironmentalSciencesE-mail:editor@jeesci.comCellproliferationofPhaeocystisglobasa,aredtidecausativemarinemicroalgainvariousphosphorus(P)-andnitrogen(N)-repleteconditions*CaiZhuoping,HuangWeiwei,Duan
2、ShunshanInstituteofHydrobiology,JinanUniversity,Guangzhou510632,ChinaAbstract:Phaeocystisglobosa,aprevalentbloom-formingmicroalgalspecieswasgrowninvariousphosphorus(P)-repleteandni--3-1-3-1-3-1-3-1trogen(N)-repleteconditions,1P1N(f/2medium):P=5×10g·L,N=7
3、5×10g·L;3P1N:P=15×10g·L,N=75×10g·L;1P3N:-3-1-3-1-3-1-3-1P=5×10g·L,N=225×10g·Land3P3N:P=15×10g·L,N=225×10g·L,anditscellgrowthwasmeasuredbyusingchloro-phyllfluorescencedeterminationandcell-countingmethods.TheresultsshowedthatgrowthcurvesofPhaeocystisglobos
4、aexposedeachnutrientconditionsexhibitedas“S-shaped”curvesthroughouttheexperiment,indicatingthemicroalgalcellsexperiencedthreegrowthstages,namelyslow-growthstage,fast-growstageandstationary-growthstage.Chlorophyllfluorescencewasaffectedobvi-ouslybythePand
5、/orNconcentrations.Significantlyhigherchlorophyllfluorescencewasobservedinthe3P1N,1P3Nand3P3N-1-1(above900μg·L)ascomparedwiththanthatin1P1N(only850μg·L),buttherewerenosignificantdifferencesinthechlorophyllfluorescenceamong3P1N,1P3Nand3P3Nconditions.Besid
6、es,remarkablyhigherspecificgrowthratewasfoundin3P3Nand-1-1-13P1Nconditions(bothabove0.77d)than1P1Nand1P3Nconditions(only0.70dand0.69drespectively).Finally,changesinthecelldensityofPhaeocystisglobosaexposedtodifferentphosphorus-repleteandnitrogen-repletec
7、onditionsintheterminationofexperimentwereconsistentwiththechangesinchlorophyllfluorescence,withtherelativelyhighercelldensityin3P1N,1P3Nand3P3Nconditionsthan1P1N.OurresultsdemonstratethathighconcentrationofPor/andNinthewaterisamajorfactorresponsibleforth
8、efastgrowthofmicroalgalcells,andthatmeasuringthechlorophyllfluorescenceinmicroalgalcellsisaquick,simple,sensi-tiveandreliablemethod,henceitshouldbeutilizedinthepredictingandmanagingredtides.Keywords:Phaeocystisglobosa;cell
