基于达托霉素亲和作用的革兰氏阳性菌检测新方法

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1、单位代码10635学号112015329001954硕士学位论文基于达托霉素亲和作用的革兰氏阳性菌检测新方法论文作者：王梦遥指导教师：付志锋教授学科专业：药物分析学研究方向：药物光谱分析提交论文日期：2018年04月10日论文答辩日期：2018年05月30日学位授予单位：西南大学中国重庆2018年05月DissertationforMasterDegreeofSouthwestUniversityNovel-detectionmethodsforGram-positivebacteriautilizingdaptomycinaffinitymechanismCandidate:Wa

2、ngMengyaoaAdvisor:Prof.FuZhifengDiscipline:PharmaceuticalAnalysisMajor:SpectroscopicpharmaceuticalAnalysisChongqing,ChinaMay2018目录摘要································································································IABSTRACT··························································

3、······························III主要缩写符号对照表···········································································VII第1章文献综述··················································································11.1选题背景及意义·········································································11

4、.2细菌检测的研究现状···································································21.2.1基于培养模式的传统检测方法················································21.2.2基于分子生物学的检测方法···················································21.2.3基于分子识别模式的检测方法················································21.3分子发光分析法···

5、·····································································101.3.1生物发光分析法·································································101.3.2荧光分析法·······································································111.4磁性微球分离技术···················································

6、··················141.5研究思路及创新点·····································································15第2章基于达托霉素亲和作用的生物发光分析法检测革兰氏阳性活菌···········172.1引言························································································172.2实验部分················································

7、·································182.2.1试剂和材料·······································································182.2.2仪器················································································192.2.3细菌的培养和计数··········