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ID:36860360
大小:2.76 MB
页数:54页
时间:2019-05-16
《叶绿素荧光检测技术及仪器的研制》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中文摘要近年来,海洋污染情况日趋严重,所形成的“赤潮”遍及世界各大洲。我国太湖、芜湖、巢湖等相继爆发“水华”。水体富营养化、水中浮游植物和藻类的大量繁殖是产生赤潮和水华的主要原因。浮游植物的过度繁殖,使得水中氧分耗尽,水中其它生物因此大量死亡。同时,藻类的快速生长,在水面形成一层膜,阻断了空气和阳光的进入,因此水质逐日变得恶臭,给人们的生产、生活和生命健康都带来了极大的危害。水中叶绿素可作为水体营养的跟踪指示器,快速检测水中的叶绿素含量可以预警水质污染。本文选用荧光测量法,它采用一定波长的光激发浮游植物的叶绿素产生荧光,通过检测产生的荧
2、光强度实现对浮游植物浓度的测定。文章简述了叶绿素荧光测量法的国内外研究现状,介绍了荧光产生的过程和荧光的光谱特性,对荧光测量方法的原理进行了认真分析和理论推导,重点描述了根据荧光信号特点设计的检测系统,最后通过实验测定了所研制仪器的线性、稳定性和重复性。综上所述,该叶绿素荧光测量仪器具有灵敏度高、无需试剂、速度快、实时性好的优点,可用于湖泊、河流、海洋等水体的实时在线检测。关键词:水华富营养化叶绿素荧光光谱ABSTRACTAtpresent,theoceanpollutionisbecomingmoreandmoreseriousand
3、RedTidesevenoccurredatallthecoastsofthecontinentsintheworld.MostlakesinChina,suchasTaihuLake,WuLake,ChaohuLakeandSOon,sufferedfromWaterBloomsinrecentyears.Theexcessivebreedingofphytoplanktonexhaustedtheoxygeninthewaterandthereforeresultedindeathofalargenumberofotheraquat
4、iclife.Atthesametime,duetotherapidgrowthofalgae,amembranelayerWasformedinthesurface,whichinterdictedtheentryoffreshairandthesunshine,hencewaterpollutionisgrowingmoreandmoreseriousdaybyday.Alloftheabovebroughtgreatharmtopeople’Sdailylife.Asanindicatorofwatereutrophication
5、,rapiddetectionofchlorophyllcontentcanpredicttheextentofwatereutrophicationandpollution.Therearelotsofwaystomeasuretheconcentrationofchlorophyllinthewater,andinthispaperweadoptthefluorescencemethod.Heredeterminatedwavelengthoflightisusedtostimulatethephytoplanktoninthewa
6、ter,andthenwecangettheconcentrationofphytoplanktonbydetectingthefluorescenceintensityofthechlorophyll.Thispaperfirstlydepictsthepresentresearchstatusofchlorophyllfluorescencemeasurementsathomeandabroadbriefly,andthenintroducestheprocessoffluorescence’Semissionandspectral
7、properties.Besidesweanalyzethetheoreticalderivationabouttheprinciplesoffluorescencemeasuringindetail.Afterthatweemphaticallydescribethedetectionsystemwhichisdesignedaccordingtothefluorescencecharacter,includingdrivingcircuitofLED,modulationanddemodulationoffluorescencesi
8、gnal,receivingcircuit,amplifiercircuit,processingcircuitanddatacommunicationmodule.Attheendofthispaper,
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