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ID:36788225
大小:832.75 KB
页数:29页
时间:2019-05-15
《聚焦超声抗小鼠早孕后肿瘤坏死因子α在子宫内膜中的表达变化研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、华中科技大学同济医学院硕士学位论文聚焦超声抗小鼠早孕后肿瘤坏死因子Q在子宫内膜中的表达变化研究研究生:王瑞玲指导老师:李红发教授华中科技大学同济医学院附属协和医院妇产科摘要目的通过了解肿瘤坏死因子n(TNF—n)在聚焦超声波终止小鼠早孕过程中在子宫内膜的表达变化,以探讨其在终止妊娠中的作用。方法采用声强12W/cm2的聚焦超声波,辐照孕小鼠第7天一侧子宫胚胎所有着床点,辐照时间90秒,分别取辐照后即刻、辐照后24h、48h、72h及辐照后第7天的子宫,对侧子宫做自身对照,固定后做光镜及电镜检查观察子宫内膜的形
2、态变化,采用免疫组化方法测定子宫内膜的肿瘤坏死因子n的表达。结果肉眼观察辐照侧子宫内小鼠胚胎停止发育,而对侧未照射的子宫内小鼠胚胎继续发育。光镜及电镜结果显示辐照后子宫内膜出现损伤并逐渐恢复,而对侧子宫内膜未见异常。免疫组化显示辐照组子宫内膜的肿瘤坏死因子a平均光密度明显高于对照组子宫内膜平均光密度,组内比较差异有显著性(P3、胎死亡外,辐照侧肿瘤坏死因子a升高,使子宫内膜微环境改变,也是导致流产发生的因素之一。关键词超声波;终止妊娠:小鼠;子宫内膜;肿瘤坏死因子华中科技大学同济医学院硕士学位论文ThevarietytumornecrosisfactoralphainendometrialofmouseafterterminationofgestationinducedbyfocusultrasonicPostgraduate:WangRuilingTutor:Prof.LiHongfaDept.ofobstet&Gynecol,U4、nionHospital,TongiiMedicalcollege,HuazhongUniversityofScienceTechnology,Wuhan,430022AbstractObjective:Toinvestigatetheexpressionoftumornecrosisfactor却haintheendometrialofmouseinthecourseofterminationofearlypregnancyinducedbyfocusultrasonic,studytherum.ornec5、rosisfactoralphaintheendingearlypregnancybyfocusultrasonic.Methods:Focusultrasonicirradiatestheimplantationsitof7-day·oldembryosofonesideofuterusbyanintensityof12W/cm2,90s.Thentakeouttheuterusesafterirradiationimmediately,24h,48h,72hand7d.Theoppositeuteruse6、sareself-contrastgroups.Observingtheuterusesmorphologicchangeafterspecimenfixationthroughopticalmicroscopyandelectronmicroscopy,determinetheexpressoftumornecrosisfactoralphaintheendometrialwiththeimmunohistochemicalmethod.Results:Macroscopicobservation,them7、ouseembryostoceasegrowthinthesideofuterusirradiatedbyfocusultrasonic,buttheembryostocontinuegrowthintheoppositesideuterus.Theresultsofopticalmicroscopyandelectronmicroscopyrevealtheendometrialisdamagedandrepairedgradually,butoppositesideendometrialisnoabnor8、malityseen.Theaveragelightdensityvalueoftumornecrosisfactoralphainthe2华中科技大学同济医学院硕士学位论文endometrialofirradiationgroupisobviouslyhigherthancontrastgroup(p<0.01).Theaveragelightdensityvalueoftumornecrosis
3、胎死亡外,辐照侧肿瘤坏死因子a升高,使子宫内膜微环境改变,也是导致流产发生的因素之一。关键词超声波;终止妊娠:小鼠;子宫内膜;肿瘤坏死因子华中科技大学同济医学院硕士学位论文ThevarietytumornecrosisfactoralphainendometrialofmouseafterterminationofgestationinducedbyfocusultrasonicPostgraduate:WangRuilingTutor:Prof.LiHongfaDept.ofobstet&Gynecol,U
4、nionHospital,TongiiMedicalcollege,HuazhongUniversityofScienceTechnology,Wuhan,430022AbstractObjective:Toinvestigatetheexpressionoftumornecrosisfactor却haintheendometrialofmouseinthecourseofterminationofearlypregnancyinducedbyfocusultrasonic,studytherum.ornec
5、rosisfactoralphaintheendingearlypregnancybyfocusultrasonic.Methods:Focusultrasonicirradiatestheimplantationsitof7-day·oldembryosofonesideofuterusbyanintensityof12W/cm2,90s.Thentakeouttheuterusesafterirradiationimmediately,24h,48h,72hand7d.Theoppositeuteruse
6、sareself-contrastgroups.Observingtheuterusesmorphologicchangeafterspecimenfixationthroughopticalmicroscopyandelectronmicroscopy,determinetheexpressoftumornecrosisfactoralphaintheendometrialwiththeimmunohistochemicalmethod.Results:Macroscopicobservation,them
7、ouseembryostoceasegrowthinthesideofuterusirradiatedbyfocusultrasonic,buttheembryostocontinuegrowthintheoppositesideuterus.Theresultsofopticalmicroscopyandelectronmicroscopyrevealtheendometrialisdamagedandrepairedgradually,butoppositesideendometrialisnoabnor
8、malityseen.Theaveragelightdensityvalueoftumornecrosisfactoralphainthe2华中科技大学同济医学院硕士学位论文endometrialofirradiationgroupisobviouslyhigherthancontrastgroup(p<0.01).Theaveragelightdensityvalueoftumornecrosis
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