流式细胞仪检测细胞凋亡操作流程

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1、流式检测细胞凋亡AAnnexinnnexinVV检检测细胞凋亡测细胞凋亡........................................................................................2实验原理................................................................................................................2实验用品................................................

2、................................................................2操作步骤................................................................................................................3AnnexinVBlocking..............................................................................................5凋

3、凋亡细胞的亡细胞的DDNANA断断裂片段分析裂片段分析.............................................................................7实验原理................................................................................................................7实验用品....................................................................

4、............................................8操作步骤................................................................................................................9BBrdUrdUFFlowlowKKitsits检检测细胞增殖测细胞增殖..............................................................................12实验原理............

5、..................................................................................................12BrdUFlowKits试剂盒.......................................................................................12结果分析......................................................................................

6、........................17流式仪器设置指南..............................................................................................18线线粒体膜电位变化检测细胞凋亡粒体膜电位变化检测细胞凋亡..........................................................................22实验原理........................................................

7、......................................................22实验用品..............................................................................................................22样本制备............................................................