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1、现代食品科技ModernFoodScienceandTechnology2015,Vol.31,No.7苦荞麦多糖对铅中毒小鼠的保护作用研究121311张季，严春临，王博奥，张鹏，侯勇，薛贵平（1河北北方学院药学系，河北张家口075000）（2河北北方学院基础医学院，河北张家口075000）（3内蒙古自治区临河人民医院医务科，内蒙古临河015000）摘要：研究了苦荞麦多糖对铅中毒小鼠的保护作用及作用机制。采用小鼠每天腹腔注射醋酸铅溶液7mg/kg，连续注射8d制备铅中毒模型，自造模第12d起各组小鼠每天灌胃相应剂量的苦荞麦多
2、糖进行治疗，连续20d。以避暗实验和Morris水迷宫实验对小鼠进行学习记忆能力和空间辨别能力的测试，采用原子分光光度法分别测定小鼠组织和血浆中的铅含量，采用紫外分光光度法测定组织及血浆中超氧化物歧化酶（Superoxidedismutase,SOD）、谷胱甘肽过氧化物酶（glutathioneperoxidase,GSH-Px）活力和丙二醛（malondialdehyde,MDA）含量，采用透射电镜观察铅中毒小鼠海马神经元超微结构。研究发现苦荞麦多糖可显著改善铅中毒小鼠的学习记忆能力和空间辨别能力，显著降低铅中毒小鼠组织和
3、血浆中的铅含量，显著提高铅中毒小鼠组织和血浆中SOD、GSH-Px活力，降低MDA含量，连续20d灌胃给药苦荞麦多糖可以显著改善铅中毒造成的海马神经元损伤。因此苦荞麦多糖可通过提高铅中毒小鼠抗氧化酶活性，清除自由基，改善铅中毒造成的损伤。关键词：苦荞麦多糖；铅中毒；自由基；水迷宫；学习记忆文章篇号：1673-9078(2015)7-12-17DOI:10.13982/j.mfst.1673-9078.2015.7.003ProtectiveEffectsofTartaryBuckwheatPolysaccharidesonL
4、ead-poisonedMice121311ZHANGJi,YANChun-lin,WANGBo-ao,ZHANGPeng,HOUYong,XUEGui-ping(1.DeptofPharmacology,HebeiNorthUniversity,Zhangjiakou075000,China)(2.BasicMedicalCollege,HebeiNorthUniversity,Zhangjiakou075000,China)(3.MedicalDepartmentofLinhepeople'shospital,TheIn
5、nerMongoliaAutonomousRegion015000,China)Abstract:ThemechanismandprotectiveeffectsofTartarybuckwheatpolysaccharides(TBP)onlead-poisonedmicewereinvestigated.Themicewereinjectedintraperitoneallywith7mg/kgleadacetatesolutiondaily,for8daystoproducethelead-poisoningmodel
6、.Afterthethmodelwasestablished,variousdosesofTBPwereadministeredviagastricinfusionbydifferentgroupsofmiceeverydayfromthe12dayon,astreatmentsfor20consecutivedays.PassiveavoidancetestandMorriswatermazetestswereusedtotesttheabilitiesoflearningandmemoryaswellasspatiald
7、iscrimination,respectively.Leadcontentintheplasmaandtissueweredeterminedbyatomicspectrometry.Theactivitiesofsuperoxidedismutase(SOD),glutathioneperoxidase(GSH-Px),andthecontentofmalondialdehyde(MDA)intissuesandplasmaofthemiceweredeterminedusingultravioletspectropho
8、tometry.Ultrastructureofmicehippocampalneuronswasobservedbytransmissionelectronmicroscopy.TheresultsshowedthatTBPcouldsignificantlyimprovethelear
