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1、生物多样性2007,15(3):292–299doi:10.1360/biodiv.060133BiodiversitySciencehttp://www.biodiversity-science.net·研究简报·应用ISSR-PCR分析蒙古栎种群的遗传多样性111232*张杰吴迪汪春蕾屈红军邹学忠杨传平1(东北林业大学生命科学学院,哈尔滨150040)2(东北林业大学林学院,哈尔滨150040)3(辽宁省林业职业技术学院,沈阳110101)摘要:本研究应用ISSR标记技术对东北地区的优势树种蒙古栎(Quercusmongolica)的25个种群遗传多样性进行了分析,
2、目的是为蒙古栎早期选择提供依据。从60条ISSR引物中筛选出10个特异性强、稳定性好的引物进行ISSR分析。共获得位点数71个,其中多态位点数56个,多态位点百分率为78.87%。PopGene分析结果表明:种群的平均多态位点百分率为45.2%,Shannon表型多样性指数(I)平均值为0.25,具有较高的遗传多样性,种群间存在一定程度的基因流(Nm为1.3818)和遗传分化(Nei’s信息多样性指数平均值为0.1068,Gst平均值为0.2657),种群内的基因多样度占总种群的73.43%,种群间占26.57%,表明蒙古栎种群的变异主要来源于种群内。结合聚类分析和地理
3、变异规律把种群划分为两个大的种群组:小兴安岭种群组和长白山种群组。以上结果可为栎属种质资源的保护和利用以及物种分化研究提供基础资料。关键词:Quercusmongolica,ISSR,遗传多样性,遗传结构GeneticdiversityanalysisofQuercusmongolicapopulationswithIn-ter-SimpleSequenceRepeats(ISSR)technique111232*JieZhang,DiWu,ChunleiWang,HongjunQu,XuezhongZou,ChuanpingYang1CollegeofLifeScie
4、nces,NortheastForestryUniversity,Harbin1500402CollegeofForestry,NortheastForestryUniversity,Harbin1500403LiaoningForestryTechnologicalCollege,Shenyang110101Abstract:Geneticdiversityoftwenty-fivepopulationsofQuercusmongolica,whichisadominanttreespeciesfromNortheastChina,wasinvestigatedbyt
5、heInter-SimpleSequenceRepeat(ISSR)technique,us-ingtenspecificandstableprimersselectedfrom60primers.Atotalof71sitesweregenerated,56ofwhich(78.87%)werepolymorphic.ThedatawereanalyzedbyPopGene.Theaveragepercentageofpolymorphiclociofpopulationswas45.2%anddemonstratedhighgeneticdiversity(thea
6、veragevalueofShannonindex(I)was0.25).Tosomeextent,bothgeneflowandgeneticdiversitywerepresentamongpopulations(Nmwas1.3818,averageNei’sindexwas0.1068,andaverageGstwas0.2657).Thegeneproportionofdiversityreached73.43%withinpopulationbutwas26.57%amongpopulations.Thisdemonstratedthatgeneticvar
7、ia-tionofQ.mongolicapopulationswasmainlywithinpopulations.Accordingtoclusteranalysisandthelawofgeographicvariation,thepopulationswereclassifiedintotwolargegroups:theXiaoxinganlinggroupandtheChangbaishangroup.TheseresultssupplementinformationforprotectionandutilizationofQ.
