基于SPR技术的传感芯片的研制及其应用

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1、北京协和医学院;中国医学科学院博士学位论文基于SPR技术的传感芯片的研制及其应用姓名：欧惠超申请学位级别：博士专业：生物医学工程指导教师：宋存先;罗昭锋20090301北京协和医学院博士学位论文研制出多种具有自主知识产权的SPR传感芯片，不仅大大降低了仪器的运行成本，而且在一定程度上提高了SPR生物传感器的检测分析能力，为SPR生物传感器的进一步应用推广以及国产SPR生物传感器的研发应用奠定了基础。此外，通过应用自主制备芯片进行多方面的分子相互作用分析和物质浓度检测，为SPR技术在新领域的应用提供了新的思路。

2、关键词：表面等离子共振，生物传感器，传感芯片，再生，生物分子相互作用II北京协和医学院博士学位论文ABSTRACTThebiosensorbasedontheprincipleofsurfaceplasmonresonance(SPR)，isasurfacesensitiveopticaldeviceformonitoringbiomolecularinteractionsatthesensorsurfaceinrealtimewithoutanylabeling．Itisbeingusedina、析deva

3、rietyofareassuchasproteomics，drugdiscovery,clinicaldiagnosis，foodanalysis，environmentalmonitoringandSOon．ThesensorchipisthecoreoftheBiacoreinstrument．SincesensorchipscannowonlybepurchasedfromBiacoreAB(Uppsala，Sweden)，whichmakesthemexpensive，manyinstrumentsa

4、reeitherusedatalowrateorsleeping．Besides，fewtypesofsensorchipsanddifficultimmobilizationofmaterialstothesensorchipsurface，arehinderingtheapplicationofSPRbiosensortoacenianextent．Inthispaper,inordertoreducethecostofsnesorchips，thepreparationandregenerationme

5、thodsoftheJ1，C1，CM5，SAandNTAsensorchipwerestudied，andtheirpreparationprocesswereperfected．Throughsubstantialexperimentalverificationaswellasanumberofresearchinstitutes’trials，theself-preparedsensorchipCanachievethesamequalityastheBiacoresensorchipandmeetthe

6、requirementsoftheexperiment．Inordertoincreasethetypesofoptionalsensorchips，areducedbovineserumalbumin(rBSA)sensorchipWasdeveloped，whichisanewsensorchipfortheBiacoreinstrumentandCanachievethesamefunctionastheCM5sensorchip．ThepreparationofrBSAsensorchipissimp

7、le，rapidandlowcost．Theatomicforcemicroscopy(AFM)characterizationoftherBSAsensorchipsurfaceandtheapplicationtodetectsulfamethoxazole(SMX)bytherBSAsensorchipusinganinhibitiveimmunoassayformat，demonstratedhomogeneousmatrixsurfaceandexcellentperformanceoftherBS

8、Asensorchip，whichisabletomeettheneedsoftheexperimentandtherequirementofquantitativeanalysisofSMX．InordertoimprovethebindingperformanceoftheNTAsensorchip，ahighermoleculardensityofanewimprovedNTAsensorch