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1、17-第五章培养基及设备的灭菌（Sterilizationofmediumandequipmentin17-fifthchapter）Thefifthchapter:sterilizationofmediumandequipmentThefirstsectionofmediumsterilizationpurpose,requirementsandmethodsFirst,definition1.DefinitionofmediumsterilizationThekillingoflivingbacteriaa
2、ndsporesfromtheculturemediumorremovingthemfromtheculturemedium.Mediumscaleindustrialliquidsterilization,killbacteriaremovalbacteriaismorecommon.2,thedifferencebetweensterilizationanddisinfectionSterilization:thekillingorremovalofallmicroorganismsintheenviron
3、ment,includingvegetativecells,bacterialsporesandspores,byphysicalorchemicalmethodsDisinfection:physicalorchemicalmethodstokillpathogenicmicroorganismsinmaterials,containersandcontainers.Two.Thepurposeofculturemediumsterilization1,theinclusioncausedbyotherbac
4、teriainthefermentationprocessoftheconsequences:Theproductionofbacteriaandbacteriaandbacteriagrowth,lossofproductioncapacity;Intheprocessofcontinuousfermentation,thegrowthrateofthehybridbacteriaisfasterthanthatoftheproducingbacteria,andtheresultisthatthebacte
5、riainthefermentationtankaredominatedbymiscellaneousbacteria;Thebacteriaandthematerial,theextractionandpurificationdifficultMicrobesdegradetargetproducts;Thebacteriawillpollutethefinalproduct,thebacteriawillpollutethefinalproduct;Duringthefermentationofsuchco
6、ntaminatedphagecanmaketheproducingbacterialysis.2,industrialspecificmeasuresinclude:(1)mediumandequipmentusedtobesterilized;(2)aerobiccultureusedinairsterilizingtreatment;(3)theequipmentshouldbetight,fermentationtankpressuretomaintaintheenvironment;(4)inthep
7、rocessoftrainingmaterialsshouldbeaddedaftersterilization;(5)usepureseedswithoutpollution.3.ThepurposeofculturemediumsterilizationKillthemicroorganismsintheculturemedium,tocreateconditionsforthesubsequentasepticfermentationprocess.4.Requirementsofmediumsteril
8、ization(1)toachievetherequirementofsterility(10-3)(2)minimizethedestructionofnutrients.Duringsterilization,thedestructionofthecomponentsoftheculturemediumiscausedbytwobasictypesofreactions:*deve
