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1、...页眉乙型肝炎肝纤维化组织差异蛋白的筛选及DDAH1的功能研究 作者：罗新华，程明亮，张权，杨勤作者单位：1.贵阳医学院附院感染科，贵州贵阳550004;2.贵阳医学院病理生理学教研室，贵州贵阳550504 【摘要】目的：对比分析乙型肝炎纤维化和非纤维化肝组织的蛋白质组表达谱的差异，探讨二甲基精氨酸二甲基氨基水解酶1(DDAH1)在肝纤维化发生发展中的功能。方法：利用双向凝胶电泳(2-DE)筛选肝纤维化和非纤维化肝组织差异表达的蛋白质，质谱鉴定差异蛋白质，Western-blot验证DDAH1的表达水平;
2、测定肝组织中DDAH1活性，不对称二甲基精氨酸(ADMA)的含量，一氧化氮合酶(NOS)活性及NO2-/NO3-比值。结果：鉴定出DDAH1等25个2倍以上的差异蛋白质;肝纤维化组织中DDAH1的活性降低，ADMA含量增加，NOS活性下降，一氧化氮(NO)含量减少。结论：乙型肝炎肝纤维化与非纤维化肝组织的蛋白质表达谱有一定差异，DDAH1有可能通过调节ADMA的代谢而影响NO合成，参与了肝纤维化的发生发展。 【关键词】肝炎，乙型，慢性;纤维化;蛋白质组学;二甲基精氨酸;二甲基氨基水解酶类 AStudyonth
3、eDifferentialProteinsandFunctionsofDDAH1in....页脚...页眉 HumanHepatitisBDerivedHepaticFibrosisTissues LUOXinhua,CHENGMingliang,ZHANGQuan,YANGQin (1.DepartmentofInfectiousDisease,TheAffiliatedHospitalofGuiyangMedicalCollege,Guiyang550004,Guizhou,China; 2.Depa
4、rtmentofPathophysiology,GuiyangMedicalCollege,Guiyang550004,Guizhou,China) [Abstract]Objective:TocompareandanalyzethedifferenceofproteomicexpressionprofilesbetweenhumanhepatitisB(HB)derivedhepaticfibrosistissuesandnon-fibrosislivertissues,andanalyzethefuncti
5、onsofdimethylaminohydrolase(DDAH1)intheformationanddevelopmentofhepaticfibrosis.Methods:Thedifferentialexpressionofproteinswerescreenedwithtwodimensionelectrophoresis(2-DE)andidentifiedwithmassspectrogramafterthetotalproteinwasextractedfromhepaticfibrosistiss
6、ueandnonfibrosislivertissue.DDAH1expressionwasverifiedwithWestern-blot.DDAH1activity,asymmetrydiethylarginine,(ADMA)concentration,nitricoxidesynthase(NOS)activity,andnitrogenmonoxidium(NO)concentrationweredetermined.Results:Twenty-fiveproteinswith2-folddiffer
7、encewereidentified.TheresultsofDDAH1bywesternblotshowedconformitywiththatby2-DE.Furtherstudyshowedthat,incomparingwiththoseofnon-fibrosislivertissue,theactivityofDDAH1wasloweredwiththeincreaseofADMAinhepaticfibrosistissues(2.04±0.33Μmol/Lvs1.16±0.21Μmol/L,...
8、.页脚...页眉P<0.01),NOSactivitylowered(4.39±0.66U/mlvs7.65±0.91U/ml,P<0.05),andtheNOcontentdecreased(55.72±11.32Μmol/Lvs96.27±17.84Μmol/L,P<0.01).Conclusions:Thereisdifferenceofproteinexpress
