犬毛色、毛色基因型及性别与导盲犬培训成功率的相关性研究

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1、■Ｉ．－一．．■密级硕：ｉ：学位论文＞、：Ｉ．屬犬毛色、毛色基因型及性别与导盲犬培训成功率的相关性研究＇－？－—作者姓名：俞剑熊指导教师；王靖宇学科专业：动物学大连医科大学中图分类号Q38密级犬毛色、毛色基因型及性别与导盲犬培训成功率的相关性研究俞剑熊计：学位论文：34页表格：5个插图：7幅指导教师：王靖宇教授申请学位级别：硕士学位学科（专业）：动物学培养单位：大连医科大学实验动物中心完成时间：二○一五年三月答辩委员会主席：独创性声明本人郑重声明：所呈交的学位论文是本人在指导教师指导下进行的研究工作及所取得

2、的研究成果。除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含为获得大连医科大学或其它教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意。学位论文作者签名：签字日期：年月日关于学位论文使用授权的说明本学位论文作者完全了解学校有关保留、使用学位论文的规定，同意学校保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权大连医科大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本学位论文属于（

3、请在以下相应方框内打“√”）：1.保密□，在年解密后适用本授权书。2.不保密□。作者签名：日期：年月日导师签名：日期：年月日目录一、摘要·············································································1（一）中文摘要····································································1（二）英文摘要································································

4、····3二、正文·············································································5（一）前言··········································································5（二）材料和方法·································································61.研究对象··············································

5、·························62.仪器与试剂····································································72.1仪器·····································································72.2试剂·····································································73.实验方法···································

6、··································73.1犬全血的采集·························································73.2犬基因组DNA的提取及验证·····································73.3特异性DNA片段的获取及验证··································83.4PCR扩增产物的纯化及验证·······································93.5质粒DNA的制备与检测···············

7、····························93.6基因型的分析·······················································103.7数据分析·····························································10（三）结果········································································