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1、JanDrenthPrinciplesofProteinX-RayCrystallographyThirdEditionWithMajorContributionfromJeroenMestersUniversityofLubeck,Germany¨JanDrenthLaboratoryofBiophysicalChemistryUniversityofGroningenNyenborgh49747AGGroningenTheNetherlandsj.drenth@rug.nlCoverillustration:CourtesyofAd
2、rianR.Ferr´e-D’Amar´eandStephenK.Burley,TheRockefellerUniversity.Thefour--helixbundlemoietyoftranscriptionfactorMax.ReproducedwithpermissionfromNature363:38–45(1993).LibraryofCongressControlNumber:2006926449ISBN-10:0-387-33334-7e-ISBN-10:0-387-33746-6ISBN-13:978-0-387-3
3、3334-2e-ISBN-13:978-0-387-33746-3Printedonacid-freepaper.C2007,1999,1994SpringerScience+BusinessMedia,LLCAllrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(SpringerScience+BusinessMedia,LLC,233SpringStreet,NewYor
4、k,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.Theuseinthispublicat
5、ionoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.987654321springer.comPrefacetotheThirdEditionInthe7yearssincethepublicationofthepreviousedition,
6、proteinX-raycrys-tallographyhasmaderapidprogress.Thiswas,toalargeextent,triggeredbythesuddengrowthofinterestinstructuralgenomics.Impressivetechnologicalad-vancesfacilitatedthisdevelopment.ItrequiredupdatingChapter2onSourcesandDetectors.Itisnolongeranexceptiontocollectafu
7、lldatasetinafewminutesandtosolvethestructureinhours.However,onebottleneckremainsandthatisthegrowthofqualitycrystals.Althoughacceleratedbyroboticsystems,itisstillatrial-and-errorprocess.Forthebenefitofthenoviceincrystallization,achapteron“PracticalProteinCrystallization”wr
8、ittenbyDr.JeroenR.Mestershasbeenadded.Anotheradditionisasectiononsingle-wavelengthanomalousdiffraction.
