Springer.Modern.Techniques.In.Neuroscience.Research.Windhorst.Johansson.1999.Springer.3540644601

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1、ContentsChapter1CytologicalStainingMethodsRobertW.BanksIntroduction.............................1Subprotocol1:Fixation,SectioningandEmbedding...........2Subprotocol2:Ultrastructure.....................9Subprotocol3:TheGolgiMethod...................13Subprotocol4:Single-CellMethods.

2、..................17References.............................23Chapter2ApplicationofDifferentialDisplayandSerialAnalysisofGeneExpressionintheNervousSystemErnoVreugdenhil,JeannettedeJongandNicoleDatsonIntroduction.............................27Subprotocol1:DifferentialDisplay:Practica

3、lApproach..........30Subprotocol2:SerialAnalysisofGeneExpression(SAGE):PracticalApproach39Subprotocol3:DigestionofcDNAwithAnchoringEnzyme........43Subprotocol4:BindingtoMagneticBeads................44Subprotocol5:AdditionofLinkers...................45Subprotocol6:TagReleasebyDigest

4、ionwithTaggingEnzyme.......46Subprotocol7:BluntingTags.....................47Subprotocol8:LigationtoDitags....................47Subprotocol9:PCRAmplificationofDitags...............48Subprotocol10:DitagIsolation....................49Subprotocol11:Concatemerisation...................

5、51Subprotocol12:CloningConcatemers.................52Subprotocol13:Sequencing......................53References.............................55Chapter3MethodsTowardsDetectionofProteinSynthesisinDendritesandAxonsJanvanMinnenandR.E.vanKesterenIntroduction.............................5

6、7Subprotocol1:InSituHybridizationofCulturedNeurons.........58Subprotocol2:InSituHybridizationattheElectronMicroscopicLevel...65Subprotocol3:Single-CellDifferentialmRNADisplay..........75Subprotocol4:FunctionalImplicationsofmRNAsinDendritesandAxons:MetabolicLabelingofIsolatedNeurite

7、s................81Subprotocol5:IntracellularInjectionofmRNA.............84References.............................87VIIIContentsChapter4OpticalRecordingfromIndividualNeuronsinCultureAndrewBullenandPeterSaggauIntroduction............................89Outline.........................

8、......102Materials........